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Updated: Feb 7, 2026

Electric Cell-substrate Impedance Sensing for the Quantification of Endothelial Proliferation, Barrier Function, and Motility
Published on: March 28, 2014
Electric Cell-Substrate Impedance Sensing (ECIS) for the analysis of shear stress effects on cell monolayers
Fernando Pesantez Torres1, Michael Detweiler2, Charles R Keese2
1Department of Nanoscale Science and Engineering, College of Nanotechnology, Science and Engineering, University at Albany, State University of New York, 257 Fuller Road, Albany, NY, 12203, USA.
Abstract:
Shear stress, a stress that acts co-planar with the cross-section of a system, profoundly influences cellular behavior and function. Understanding how cells respond to shear stress is critical for advancing research in vascular biology, tissue engineering, and cancer metastasis. On the other hand, Electric Cell-Substrate Impedance Sensing (ECIS) is a powerful tool for real-time, label-free monitoring of cellular behavior. This review examines the application of combining ECIS and flow systems to study, in real-time, the effects of shear stress on cell monolayers, such as the impact on barrier function. It highlights its advantages, the various experimental setups, and key experimental findings.
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