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Updated: Feb 7, 2026
08:38
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
Published on: November 8, 2015
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A high-throughput detection platform for dried blood spots constructed with DESI-MS/MS
Siqi Gao1, Jinhui Zhao2, Yue Guan3
1Department of Sanitary Inspection, School of Public Health, Harbin Medical University, Harbin, 150086, P. R. China; Shanghai Jiao Tong University Affiliated Sixth People's Hospital South Campus, Shanghai, 201499, P. R. China.
Analytica Chimica Acta
|February 5, 2026
Summary
This study optimizes methods for analyzing dried blood spots (DBS) using desorption electrospray mass spectrometry (DESI-MS/MS). The developed protocols enable accurate detection of metabolic changes, demonstrating practicality for clinical applications like newborn screening.
Area of Science:
- Metabolomics
- Analytical Chemistry
- Biochemistry
Background:
- Dried blood spot (DBS) technology offers advantages in blood collection and metabolite preservation.
- DBS has potential applications in newborn screening and therapeutic drug monitoring.
- Desorption electrospray mass spectrometry (DESI-MS) is a rapid technique, but its performance for DBS needs evaluation.
Purpose of the Study:
- To develop and optimize protocols for analyzing metabolic profiles from DBS using DESI-MS/MS.
- To compare the performance of DESI-MS/MS with traditional UPLC-MS/MS for DBS analysis.
- To assess the practicability of the developed methods for detecting metabolic changes.
Main Methods:
- Optimized extraction solvent (ACN:H2O), volume (300 μL), and method (ultrasonic) for DBS.
- Optimized DESI-MS/MS parameters including spray solvent (MeOH:H2O), spray velocity, needle angle, and capillary voltage.
- Applied optimized methods to analyze metabolic profiles before and after consuming sugar-sweetened soy milk.
Main Results:
- Achieved consistent data, broad metabolite coverage, and acceptable reproducibility with DESI-MS/MS.
- Successfully detected differential metabolites in subjects after consuming sugar-sweetened soy milk.
- Demonstrated good performance of DESI-MS/MS compared to UPLC-MS/MS.
Conclusions:
- Two optimized protocols for DBS metabolic profiling were established.
- The optimized DESI-MS/MS method shows comparable or superior performance to UPLC-MS/MS.
- The developed protocols are practical for detecting differential metabolic changes in DBS samples.
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