Low-cost, tape-sealed, PDMS-molded devices using 3D printing for cell adhesion under flow

Microfluidics offer a useful platform for cell studies by coupling enhanced fluidic control with real-time imaging, but traditional fabrication methods require expensive equipment and advanced training. In this work, the fabrication and implementation of a low-cost and simple-to-use microfluidic device for cell studies were completed using digital light processing resin 3D printing to produce molds for polydimethylsiloxane (PDMS) devices. Contact angle analysis assessed surface characteristics during fabrication. Tape was used to reversibly seal devices, permitting reuse without damage or adhesion loss. Additionally, tape-sealed devices could withstand an excess of 4 times the internal pressure compared to PDMS devices reversibly sealed on glass. A treatment solution was developed to enable adherent culture of U-87 human glioblastoma cells on the adhesive surface and was confirmed via fluorescence-based viability and morphology monitoring. A miniature incubator, programmable fluidic system, and heating pad were incorporated to maintain conditions for long-term experiments and continuous flow. By employing this unique fabrication method, design prototyping and performance optimization can be rapidly completed for bioanalytical applications of microfluidic-based cell studies. This work provides a foundation, especially for those unfamiliar with microfluidics, to develop microfluidic platforms for cellular analysis using an accessible and less expensive approach.