Soaking Up Success: Sponge-Assisted Nanoparticle Transfection

Current cell transfection systems face efficiency limitations, particularly for challenging cell types. We present a sponge-assisted transfection method enabling efficient mRNA transfection at nanogram-scale doses-a several orders of magnitude-reduction compared to the microgram quantities typically required by lipid-based and membrane-disruption methods. This approach achieves robust, dose-dependent transfection across difficult-to-transfect cell types, establishing broad applicability for both basic research and therapeutic use.