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Updated: Feb 7, 2026

Myocardial Infarction and Functional Outcome Assessment in Pigs
Published on: April 25, 2014
Different modes of engagement with the nucleosome acidic patch yield distinct functional outcomes
Ujani Chakraborty1, Emma Christina Saccone2, Grisel Cruz-Becerra3
1Weill Cornell Medicine, Department of Pathology and Laboratory Medicine, New York, NY 10065, USA.
None:
The nucleosome acidic patch is a hub of coordinated engagement by proteins that regulate genomic function. Here we report that S. cerevisiae Dot5 contains an arginine-rich HMGN-like motif that mediates nucleosome acidic patch binding and is required for the cell growth, DNA repair and heterochromatin defects exhibited when the protein is overexpressed. The heterologous expression of camelid single chain antibodies to the nucleosome acidic patch confers a similar range of phenotypes, with the most severe observed when an 'arginine-anchor' mode of binding analogous to many endogenous factors is employed. This highlights a delicate balance between nucleosome acidic patch interactors critical for normal cellular functions and dysregulated in disease.
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