Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nuclear Export01:42

Nuclear Export

5.0K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.0K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

5.5K
5.5K
The Electromagnetic Spectrum02:37

The Electromagnetic Spectrum

65.4K
The electromagnetic spectrum consists of all the types of electromagnetic radiation arranged according to their frequency and wavelength. Each of the various colors of visible light has specific frequencies and wavelengths associated with them, and you can see that visible light makes up only a small portion of the electromagnetic spectrum. Because the technologies developed to work in various parts of the electromagnetic spectrum are different, for reasons of convenience and historical...
65.4K
Export of Mitochondrial and Chloroplast Genes02:19

Export of Mitochondrial and Chloroplast Genes

4.2K
A eukaryotic cell can have up to three different types of genetic systems: nuclear, mitochondrial, and chloroplast. During evolution, organelles have exported many genes to the nucleus; this transfer is still ongoing in some plant species. Approximately 18% of the Arabidopsis thaliana nuclear genome is thought to be derived from the chloroplast’s cyanobacterial ancestor, and around 75% of the yeast genome derived from the mitochondria’s bacterial ancestor. This export has occurred...
4.2K
IR Spectrum01:19

IR Spectrum

2.2K
When infrared (IR) radiation passes through a molecule, the bonds stretch or bend by absorbing the radiation. This absorption creates the molecule's absorption spectrum, which is the plot of its percentage transmittance versus wavenumber.
Transmittance is defined as the ratio of the radiant power passing through a sample to that from the radiation's source. Multiplying the transmittance by 100 gives the percent transmittance (%T), which varies between 100% (no absorption) and 0%...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

SLC25A3 exports mitochondrial copper to metalate cytochrome <i>c</i> oxidase and prevent cuproptosis.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Tumor CTR1 Expression and Systemic Copper Dynamics Converge on a Copper Axis in High-Grade Triple-Negative Breast Cancer.

Cancer research communications·2026
Same author

A cysteine-rich domain of the <i>Cryptococcus neoforman</i>s Cuf1 transcription factor is required for high copper stress sensing and fungal virulence.

mBio·2026
Same author

Metal homeostasis is remodeled in response to different quiescence triggers.

bioRxiv : the preprint server for biology·2026
Same author

A redox- and proton-coupled inner membrane transporter mediates copper import to the bacterial cytoplasm.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A broad-spectrum inhibitor of copper-exporting P<sub>1B</sub>-type ATPases.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Feb 7, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

14.4K

A First-In-Class Broad Spectrum Inhibitor of Copper Exporting P1B-type ATPases.

Vinit C Shanbhag1,2, Samuel Anakpeba-Dinguyella1,2, Nikita Gudekar1

  • 1Bond Life Sciences Center, University of Missouri, Columbia, MO 65211, USA.

Biorxiv : the Preprint Server for Biology
|February 6, 2026
PubMed
Summary
This summary is machine-generated.

Researchers discovered MKV3, a novel inhibitor for copper-transporting ATPases essential for cellular copper balance. This first-in-class molecule targets a conserved copper entry site, offering a new tool for various biological applications.

Keywords:
Biological SciencesCell BiologyP1B-type ATPasesantimicrobial coppercopper pumpscopper transport inhibitioncuproptosis

More Related Videos

[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
09:12

[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst

Published on: May 21, 2019

9.9K
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

10.4K

Related Experiment Videos

Last Updated: Feb 7, 2026

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses
11:34

High-throughput Screening for Broad-spectrum Chemical Inhibitors of RNA Viruses

Published on: May 5, 2014

14.4K
[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst
09:12

[DPEPhosbcpCu]PF6: A General and Broadly Applicable Copper-Based Photoredox Catalyst

Published on: May 21, 2019

9.9K
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
10:28

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors

Published on: August 17, 2019

10.4K

Area of Science:

  • Biochemistry and Molecular Biology
  • Biophysics
  • Pharmacology

Background:

  • Copper-transporting ATPases (P-type ATPases) are crucial for cellular copper homeostasis, involved in copper export and metalloenzyme synthesis.
  • Despite their importance in human diseases, specific high-affinity small-molecule inhibitors for these ATPases were previously unavailable.

Purpose of the Study:

  • To identify and characterize the first small-molecule inhibitor of copper-transporting P-type ATPases.
  • To explore the conserved druggable vulnerability within the copper entry site of these essential transporters.

Main Methods:

  • In silico docking against Xenopus ATP7B structure to identify potential inhibitor binding sites.
  • Biochemical assays to determine MKV3 binding affinity (nanomolar) to human ATP7A and ATP7B.
  • Functional assays in Escherichia coli (CopA ATPase) to assess inhibition of ATPase activity and copper transport.

Main Results:

  • MKV3 was identified as a first-in-class inhibitor, binding human ATP7A/B with nanomolar affinity and targeting a conserved Cu+ entry site.
  • MKV3 selectively inhibited E. coli CopA ATPase activity and copper transport by blocking chaperone-mediated copper delivery.
  • A single P-domain residue was found to govern MKV3 affinity and potency across diverse species, from bacteria to mammals.

Conclusions:

  • MKV3 represents a novel, broadly active chemical tool for modulating copper homeostasis by targeting a conserved, druggable vulnerability in Cu+-ATPases.
  • The findings establish a new pharmacological approach for targeting copper transport, with potential applications in antimicrobial therapy and copper-related diseases.