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Genomic Characterization of 30 Lytic Klebsiella pneumoniae Bacteriophages

Daniel Mora, Trisha Gryder, Jordyn Michalik-Provasek

    Biorxiv : the Preprint Server for Biology
    |February 6, 2026
    PubMed

    Insights

    Thirty bacteriophages were studied as potential treatments for carbapenem-resistant Klebsiella pneumoniae (CR-KP). These phages inhibited bacterial growth and lacked harmful genes, showing promise as antimicrobial alternatives.

    Area of Science:

    • Microbiology
    • Genomics
    • Public Health

    Background:

    • Antimicrobial resistance, particularly carbapenem resistance in Klebsiella pneumoniae, is a significant public health threat due to limited treatment options.
    • Bacteriophages (phages) are emerging as a promising alternative antimicrobial strategy against multi-drug resistant pathogens.

    Purpose of the Study:

    • To characterize lytic bacteriophages targeting carbapenem-resistant Klebsiella pneumoniae (CR-KP).
    • To evaluate the effectiveness and safety of these phages as potential antimicrobial agents.

    Main Methods:

    • Isolation and characterization of 30 lytic bacteriophages from environmental sources.
    • Genomic sequencing and bioinformatic analysis of phage genomes.
    • Assessment of phage virulence, antibiotic resistance genes, and host range on various media against CR-KP isolates.

    Main Results:

    • Genomic analysis revealed a diverse phage collection across 9 families and 13 genera, with genome sizes from 39-349 kbp.
    • All characterized phages demonstrated the ability to inhibit bacterial growth.
    • No virulence or antibiotic resistance genes were detected in the phage genomes.
    • Broad host range phages exhibited variable infectivity depending on the growth medium.

    Conclusions:

    • The study identified candidate phages for potential development as antimicrobial agents against CR-KP.
    • Understanding phage-host dynamics, especially for non-capsule phages targeting Klebsiella pneumoniae, remains complex.

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