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Related Concept Videos

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Dipeptidyl peptidase 4 (DPP-4) is a serine protease widely distributed in the body. It's involved in the inactivation of GLP-1 and GIP hormones, which are crucial for insulin regulation. DPP-4 inhibitors, such as sitagliptin (Januvia), saxagliptin (Onglyza), linagliptin (Tradjenta), alogliptin (Nesina), and vildagliptin (Galvus), help increase the proportion of active GLP-1, enhancing insulin secretion. These inhibitors work by competitively binding to DPP-4. This binding causes a...
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Labeling DNA Probes03:31

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Related Experiment Video

Updated: Feb 7, 2026

Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
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Prp8 Intein-Targeted Fluorescent Probe for Specific Detection of Cryptococcus neoformans and Inhibitor Screening.

Ting Guo1,2, Fang Wen1,2, Yulin Feng1

  • 1College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China.

Analytical Chemistry
|February 6, 2026
PubMed
Summary

A new fluorescent probe, H2, specifically detects Cryptococcus neoformans (C. neoformans) infections. This probe aids in rapid diagnosis and screening of antifungal drugs, crucial for global health.

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Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans
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Last Updated: Feb 7, 2026

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Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans
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Biolistic Transformation of a Fluorescent Tagged Gene into the Opportunistic Fungal Pathogen Cryptococcus neoformans

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Area of Science:

  • Medical Mycology
  • Biochemistry
  • Chemical Biology

Background:

  • Cryptococcus neoformans (C. neoformans) infections are a major global health concern, particularly for immunocompromised individuals.
  • Developing rapid, cost-effective diagnostics for C. neoformans is challenging, especially in resource-limited settings.
  • The Prp8 intein in C. neoformans is a promising target for selective diagnostic probes and antifungal therapies.

Purpose of the Study:

  • To develop novel fluorescent probes for the selective detection of C. neoformans.
  • To evaluate the efficacy of these probes in vitro and in vivo.
  • To establish a rapid screening method for Prp8 intein inhibitors.

Main Methods:

  • Synthesis of environment-sensitive fluorescent probes incorporating Prp8 intein inhibitors.
  • Evaluation of probe selectivity against Candida species.
  • Dual-staining assays combining capsule staining and intracellular Prp8 intein labeling.
  • In vivo imaging of fungal infections in a Galleria mellonella model.
  • Assessing the dose-dependent relationship between fluorescence intensity and inhibitor activity.

Main Results:

  • Probe H2 demonstrated specific detection of C. neoformans with minimal cross-reactivity with Candida species.
  • Dual-staining significantly enhanced diagnostic accuracy.
  • Successful fluorescence imaging of C. neoformans infections in vivo using the Galleria mellonella model.
  • A rapid (2-hour) screening assay for Prp8 intein inhibitors was established based on fluorescence intensity.

Conclusions:

  • Fluorescent probe H2 offers a selective and sensitive method for C. neoformans detection.
  • The developed probes and dual-staining approach can improve the diagnosis of cryptococcosis.
  • The probe facilitates efficient in vivo imaging and rapid screening of novel antifungal agents targeting the Prp8 intein.