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Updated: Feb 7, 2026

Author Spotlight: A Comprehensive Protocol for Acinetobacter Biofilm Quantification, Assessment, and Visualization
Published on: August 4, 2023
Acinetobacter-the bad, the ugly, but also the good!
Santiago Castillo-Ramírez1, Rafael López-Sánchez1, Humberto Peralta1
1Programa de Genómica Evolutiva, Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Cuernavaca, México.
Abstract:
The genus Acinetobacter is vast and diverse regarding its hosts. However, it is best known as an opportunistic pathogen that causes hard-to-treat nosocomial infections. Yet, some species of the genus can be beneficial for some hosts. Such is the case of Acinetobacter calcoaceticus, which can have a significant impact on tomato plants, as was recently shown in a paper by Robertson et al. (S. Robertson, A. Mosca, S. Ashraf, A. Corral, et al., mSphere 11:e00842-25, 2026, https://doi.org/10.1128/msphere.00842-25). Importantly, that study also exemplifies how metagenomics in general, but metagenome-assembled genomes in particular, can be employed to understand the functional specialization and identity of the bacterial species dwelling in particular environments.
Insights
Acinetobacter calcoaceticus benefits tomato plants, challenging its reputation as solely a harmful pathogen. This study highlights metagenomics for understanding bacterial roles in different environments.
Area of Science:
- Microbiology
- Plant Science
- Genomics
Background:
- The genus *Acinetobacter* comprises diverse species, often recognized as opportunistic pathogens causing nosocomial infections.
- Some *Acinetobacter* species can exhibit beneficial interactions with specific hosts.
Purpose of the Study:
- To investigate the impact of *Acinetobacter calcoaceticus* on tomato plants.
- To demonstrate the utility of metagenomics, specifically metagenome-assembled genomes, in characterizing bacterial communities and their functions.
Main Methods:
- Metagenomic analysis was employed to study bacterial communities associated with tomato plants.
- Metagenome-assembled genomes (MAGs) were reconstructed to identify and analyze bacterial species and their potential functions.
Main Results:
- The study identified *Acinetobacter calcoaceticus* as a species with a significant impact on tomato plants, suggesting a beneficial role.
- Metagenomics and MAGs proved effective in deciphering the functional specialization of bacteria within the plant environment.
Conclusions:
- *Acinetobacter calcoaceticus* can play a beneficial role for tomato plants, expanding our understanding of the genus's ecological significance.
- Metagenome-assembled genomes are a powerful tool for functional and taxonomic characterization of microbial communities in environmental studies.
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