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Published on: May 2, 2017
A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency
Yuji Miyatake1, Takeshi Kamakura2, Tomokatsu Ikawa3
1Department of Innovative Medical Science, Tokai University School of Medicine, Isehara, Japan.
None:
Accumulated studies have reported that hematopoietic differentiation was primarily regulated by transcription factors. Early B cell factor 1 (EBF1) is an essential transcription factor for B lymphopoiesis. Contrary to the canonical notion, we found that a single miRNA, miRNA-195 (Mir195) transduction let Ebf1-deficient hematopoietic progenitor cells (HPCs) express CD19, carry out V(D)J recombination and class switch recombination, which implied that B cell matured without EBF1. A part of the mechanism was caused by FOXO1 accumulation via inhibition of FOXO1 phosphorylation pathways in which targets of Mir195 are enriched. These results suggested that some miRNA transductions could function as alternatives to transcription factors.
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