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Updated: Feb 8, 2026

In Vitro Analysis of E3 Ubiquitin Ligase Function
Published on: May 14, 2021
The dynamics and functional mechanisms of H2B mono-ubiquitination
Yiqing Li1, Tianling Ma2, Jinhua Jiang3
1State Key Laboratory of Rice Biology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
Abstract:
H2B mono-ubiquitination (ub1) is an important histone modification attaching a ubiquitin moiety to the small histone H2B and changing the biochemical features of the chromatin. The dynamic equilibrium between H2B ub1 and deubiquitination (deub1) has been shown to affect nucleosome stability, nucleosome reassembly and higher chromatin structure. The above changes mediated by H2B ub1 regulate transcription activation and elongation, and play key roles in multiple molecular and biological processes including growth, development, pathogenesis and aging. In this review, we summarize our current knowledge in regulation of H2B ub1/deub1 equilibrium, and how this modification affects chromatin dynamics and gene expressions. We also discuss the roles of H2B ub1/deub1 cycle in plant-pathogen interactions, and point out the questions that remain to be resolved in future studies.
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