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Updated: Feb 8, 2026

Virus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration
Published on: April 27, 2018
Protocol for non-invasive delivery of CRISPR RNPs via virus-like particles for mouse model generation
Da Eun Yoon1, Jiyun Yang2, Tae Yeong Jeong3
1Department of Convergence Medicine, Korea University College of Medicine, Seoul 02708, Republic of Korea; Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Republic of Korea; Transgenic Core Facility, Max-Planck Institute of Biochemistry, 82152 Martinsried, Germany.
Abstract:
CRISPR-virus-like particle (VLP)-induced targeted mutagenesis (CRISPR-VIM) enables genome editing in mouse embryos through non-invasive delivery of CRISPR ribonucleoproteins (RNPs) via VLPs, eliminating the need for physical manipulation and specialized expertise. We detail protocols for VLP production, titration, and treatment for diverse genome edits. This protocol is compatible with zygotes and in vitro fertilization (IVF)-derived embryos via simple co-culture, facilitating high-efficiency and heritable mutations with minimized off-target effects, independent of specialized equipment and conducive to reduced animal use. For complete details on the use and execution of this protocol, please refer to Jeong et al.1.
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