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Published on: June 28, 2024
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Quantum Spin Detection in Microfiltration Immunoassays for Ultrasensitive and High-Throughput Diagnostics
Trong-Nghia Le1, Xuan Mai Lam1, Yi-Xiu Tang1
1Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 106, Taiwan.
Analytical Chemistry
|February 6, 2026
Summary
This study introduces a quantum-enabled microfiltration immunoassay (QEMFIA) for rapid, sensitive healthcare diagnostics. This novel quantum sensing method achieves high-throughput detection of disease markers with exceptional accuracy.
Area of Science:
- Quantum Technology
- Biotechnology
- Nanotechnology
Background:
- Current diagnostic methods face limitations in sensitivity and throughput.
- There is a need for advanced analytical techniques in healthcare diagnostics.
- Quantum sensing offers novel approaches for biological detection.
Purpose of the Study:
- To develop a quantum-enabled microfiltration immunoassay (QEMFIA) for sensitive and high-throughput diagnostics.
- To integrate quantum sensing with microfiltration for enhanced antigen detection.
- To demonstrate the potential of quantum technologies in transforming healthcare diagnostics.
Main Methods:
- Development of QEMFIA integrating dot blot and ELISA principles.
- Utilizing fluorescent nanodiamonds (FNDs) with nitrogen-vacancy centers as quantum reporters.
- Employing magnetically modulated fluorescence (MMF) for background-free detection.
- Performing assays on a 24-well microfiltration manifold for high-throughput analysis.
- Direct analysis of retained FNDs on a nitrocellulose membrane via MMF.
Main Results:
- Achieved rapid, sensitive, and quantitative detection of clinically relevant antigens.
- Demonstrated limits of detection below 100 fM for C-reactive protein and interleukin-6 within 1 hour.
- Validated compatibility with standard laboratory workflows and 96-well plates.
- Showcased potential for integration with automation platforms for broad antigen analysis.
Conclusions:
- QEMFIA represents a transformative approach to healthcare diagnostics.
- Quantum sensing with FNDs offers a powerful tool for sensitive and high-throughput biomarker detection.
- The developed method holds significant promise for advancing medical diagnostics and research.
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