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Tracking Metabolic Responses to Citalopram in Colon Cells with Raman Spectroscopy
Karolina Beton-Mysur1, Beata Brozek-Pluska1
1Faculty of Chemistry, Institute of Applied Radiation Chemistry, Laboratory of Laser Molecular Spectroscopy, Lodz University of Technology, Wroblewskiego 15, 93-590 Lodz, Poland.
Analytical Chemistry
|February 6, 2026
Summary
The antidepressant citalopram alters metabolism in colon cells, particularly cancerous ones, affecting lipid droplets and endoplasmic reticulum. Normal cells show adaptive responses, highlighting citalopram
Area of Science:
- Cellular Metabolism
- Pharmacology
- Biochemistry
Background:
- Citalopram, an antidepressant, is known for selective serotonin reuptake inhibition.
- Its effects extend beyond the brain, influencing cellular metabolism.
- Understanding citalopram's colonic effects is vital for the colon-brain axis and cancer research.
Purpose of the Study:
- To investigate citalopram-induced metabolic changes in normal and cancerous human colon cells.
- To analyze citalopram's transformation and differential handling by various colon cell types.
- To assess the impact on cellular organelles like the endoplasmic reticulum and lipid droplets.
Main Methods:
- Utilized Raman spectroscopy and imaging for label-free biochemical analysis.
- Compared metabolic responses in cancerous Caco-2 and LoVo cells versus normal CCD-18Co cells.
- Quantified changes in Raman band intensity ratios indicative of biochemical alterations.
Main Results:
- Citalopram induced significant metabolic alterations in cancerous colon cells, including changes in lipid, nucleic acid, and protein ratios.
- Observed reductions in endoplasmic reticulum and lipid droplet areas in cancerous cells, suggesting disrupted homeostasis.
- Normal CCD-18Co cells exhibited increased lipid droplet accumulation, indicating a potential adaptive detoxification response.
Conclusions:
- Raman spectroscopy effectively monitors drug-induced metabolic responses at the subcellular level.
- Citalopram differentially impacts metabolic pathways in normal versus cancerous colon cells.
- The findings provide insights into citalopram's localized effects and cellular stress responses in the colon.
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