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Simplified Human Neutrophil Extracellular Traps NETs Isolation and Handling
Published on: April 16, 2015
Multimetal exposure and its association with neutrophil extracellular traps (NETs) markers and prothrombotic
Ana Karen González Palomo1, Juan Diego Cortés García2, Kelvin Saldaña Villanueva3
1Coordination for the Innovation and Application of Science and Technology (CIACYT), Autonomous University of San Luis Potosi, San Luis Potosi, Mexico; Faculty of Chemical Science, Autonomous University of San Luis Potosi, San Luis Potosi, Mexico.
Abstract:
This study evaluated the association between multimetal exposure and neutrophil extracellular traps (NETs) and prothrombotic biomarkers in quarry workers, brickyard makers, and waste recyclers. A cross-sectional study was conducted in 91 adults. Urinary concentrations of seven metals and serum NETs and prothrombotic markers were quantified. Correlation analysis and PCA were used to identify exposure-response patterns. Waste recyclers had the highest Al and Hg levels, while brick makers showed the highest As and Zn concentrations. NETs biomarkers varied across scenario: neutrophil elastase (NE) and citrullinated histone (H3Cit) were elevated in brick makers, whereas circulating DNA was highest in quarry workers. PCA indicated that Cr-Ni-Al exposure aligned with NETs component scores, and correlations showed positive associations of Hg and As with NE, H3Cit, and myeloperoxidase (MPO). Higher As, Hg, and Zn were also associated with elevated platelets and D-dimer. Overall, multimetal exposure modulated NETs activity and early prothrombotic response.
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