Related Experiment Video

Updated: Feb 8, 2026

Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description
07:51

Effects of Allogeneic Platelet-Rich Plasma PRP on the Healing Process of Sectioned Achilles Tendons of Rats: A Methodological Description

Published on: March 19, 2018

9.7K

Distinct microRNA signatures in Platelet-Rich Plasma-Derived extracellular vesicles predict healing outcomes in

Yu-Chi Tsai1, Shu-Yu Wu2, Chien-Ju Wu3

  • 1Division of Plastic and Reconstruction Surgery, Department of Surgery, Tri-service General Hospital, National Defense Medical University, Taipei, Taiwan; Burn Center, Department of Surgery and Department of Critical Medicine, Tri-Service General Hospital, National Defense Medical University, Taipei, Taiwan; Division of Traumatology, Department of Surgery, Tri-Service General Hospital, National Defense Medical University, Taipei, Taiwan.

Diabetes Research and Clinical Practice
|February 6, 2026
PubMed
Abstract

Insights

Platelet-rich plasma extracellular vesicles (PRP-EVs) show variable efficacy for diabetic foot ulcers (DFUs). Specific microRNAs (miRNAs) within PRP-EVs predict healing outcomes, enabling personalized regenerative medicine for DFU treatment.

Area of Science:

  • Regenerative Medicine
  • Molecular Biology
  • Wound Healing Research

Background:

  • Diabetic foot ulcers (DFUs) are challenging due to poor healing.
  • Platelet-rich plasma (PRP) therapy for DFUs has inconsistent results.
  • MicroRNAs (miRNAs) within PRP-derived extracellular vesicles (PRP-EVs) may explain this variability.

Purpose of the Study:

  • Investigate the role of miRNA cargo in PRP-EVs on DFU healing.
  • Identify specific miRNAs as predictors of therapeutic efficacy.
  • Assess the potential for personalized regenerative strategies.

Main Methods:

  • Isolated autologous PRP-EVs from 10 DFU patients.
  • Quantified wound healing-associated miRNAs using qRT-PCR.
  • Monitored clinical wound closure and evaluated keratinocyte migration in vitro.

Main Results:

  • PRP treatment achieved ~90% wound closure by 6 weeks.
  • Heterogeneous miRNA expression was observed in PRP-EVs.
  • High miR-20a-5p and miR-21-5p correlated with poor healing; high miR-223-3p correlated with accelerated healing.

Conclusions:

  • PRP-EV miRNA composition critically determines therapeutic efficacy.
  • miR-20a-5p and miR-21-5p are negative predictive biomarkers for DFU healing.
  • miR-223-3p indicates a favorable prognosis, enabling personalized PRP therapy.
Keywords:
BiomarkersDiabetic foot ulcerExtracellular vesiclesMicroRNAPlatelet-rich plasmaWound healing

More Related Videos

Platelet-Rich Plasma Lysate for Treatment of Eye Surface Diseases05:57

Platelet-Rich Plasma Lysate for Treatment of Eye Surface Diseases

Published on: August 2, 2022

5.1K
Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis14:38

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Published on: January 4, 2019

19.4K

Related Concept Videos

Predicting Reaction Outcomes02:24

Predicting Reaction Outcomes

Kinetics describes the rate and path by which a reaction occurs. In contrast, thermodynamics deals with state functions and describes the properties, behavior, and components of a system. It is not concerned with the path taken by the process and cannot address the rate at which a reaction occurs. Although it does provide information about what can happen during a reaction process, it does not describe the detailed steps of what appears on an atomic or a molecular level. On the other hand,...
10.9K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.0K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
18.9K
Muscles of the Leg that Move the Foot and Toes01:28

Muscles of the Leg that Move the Foot and Toes

The human leg comprises an intricate system of muscles that facilitate the movement of feet and toes. Within this system, the muscles are categorized into the anterior, lateral, and posterior compartments, each with a unique set of muscles carrying out specific functions.
Anterior Compartment
The anterior compartment includes muscles that contribute to the dorsiflexion of the foot. This compartment houses the tibialis anterior, extensor hallucis longus, and extensor digitorum longus muscles....
4.1K
Predicting Molecular Geometry02:27

Predicting Molecular Geometry

VSEPR Theory for Determination of Electron Pair Geometries
46.0K