Related Experiment Video
Updated: Feb 8, 2026

Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Published on: July 23, 2017
Resistance to ivermectin, doramectin and moxidectin in Rhipicephalus microplus isolates from Rio Grande do Sul,
Marcelo Becker1, Tiago Gallina2, Jose Reck1
1Instituto de Pesquisas Veterinárias Desidério Finamor (IPVDF), 6000. Eldorado do Sul, Estrada do Conde 92990-000, Brazil.
Abstract:
The Rio Grande do Sul state, plays a major role in the livestock industry of Brazil, concentrating 10 % of the country's herd of cattle. The parasitism of cattle by Rhipicephalus microplus, causes great economic losses to the producers in the area. The resistance of R. microplus to macrocyclic lactones class has been detected in Rio Grande do Sul, Brazil since 2001. Side-resistance (i.e. resistance to one compound accompanied by resistance to other members of the chemical group) among the drugs of this class is an important condition, considering its implications in resistance management and cattle tick control strategies using these acaricides. The aim of this study was to evaluate the occurrence of side-resistance among ivermectin (IVM), doramectin (DOR), and moxidectin (MOX) in R. microplus from different locations of Rio Grande do Sul state, southern Brazil. Engorged female ticks were sampled in 30 different premises and their larval progeny were tested with the larval immersion test. A macrocyclic lactone resistant reference strain (IPVDF strain) was also submitted to the assays. Mortality data was submitted to probit analysis to calculate the median lethal concentrations (LC50) for each acaricide. Resistance ratios (RR) were calculated in relation to the Porto Alegre strain, being considered resistant the populations with RR ≥ 2. Fisher's test was used to determine the correlation of resistance among the drugs tested. Among the 30 isolates tested, 57 % (n = 17) were resistant to ivermectin (RR: 2.06 to 17.81); 47 % (n = 14) resistant to doramectin (RR: 2.07 to 19.03) and 30 % (n = 9) resistant to moxidectin (RR: 2.00 to 17.28). Nine samples were susceptible to all three acaricides. There was association of the occurrence of resistance between IVM and DOR (p = 0.02), between IVM and MOX (p = 0.015) and between DOR x MOX (p = 0.019). This is the first study to demonstrate side-resistance among drugs of the macrocyclic lactones family of anti-parasitic drugs in R. microplus from south Brazil. The results obtained reinforce the importance of acaricide resistance detection with laboratory bioassays and have an impact on cattle tick control strategies, since side-resistance among the macrocyclic lactones limits the availability of drugs to be used.
More Related Videos
Related Concept Videos
Resistivity
Resistance
Equivalent Resistance
Resistance and Conductance
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
Rolling Resistance
For instance, imagine a hard cylinder rolling on a comparatively soft surface. The cylinder's weight compresses the surface beneath it. As the cylinder moves, the material in front of it slows down due to...
Vascular Resistance
The primary determinants of vascular resistance are vessel diameter, blood viscosity, and vessel length. Among these, vessel diameter plays the most significant role due to the fourth power relationship described by...

