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Updated: Feb 8, 2026

Patterning Bioactive Proteins or Peptides on Hydrogel Using Photochemistry for Biological Applications
Published on: September 15, 2017
The novel DPP-IV inhibitory peptides derived from goat milk: Purification, identification, bioactivities verification
Binsong Han1, Cunzheng Zhang2, Lina Zhang3
1Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
Abstract:
Goat milk proteins are a rich source of bioactive peptides with various health benefits. To harness this potential, the primary objective of this study was to isolate and characterize novel DPP-IV inhibitory peptides from goat milk protein hydrolysates produced via simulated gastrointestinal digestion. Following fractionation by ultrafiltration and preparative RP-HPLC, twelve peptides were identified from the most active fraction (F2) using UPLC-MS/MS. Among these, IPPK exhibited the most potent DPP-IV inhibitory activity, with an IC50 value of 120.17 ± 7.18 μM. Furthermore, in-vivo studies demonstrated that IPPK effectively lowered blood glucose levels in mice, which may be associated with DPP-IV inhibition and subsequent increases in GLP-1 levels. Molecular docking analysis indicated that IPPK interacts with key residues (Arg125, Glu205, Glu206, Tyr547, and His740) within the DPP-IV active site, supporting the proposed inhibitory mechanism. These findings suggest that goat milk-derived peptides, particularly IPPK, hold promise as potential therapeutic potential for diabetes management and warrant further investigation.
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