Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Responses to Salt Stress02:02

Responses to Salt Stress

14.6K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.6K
The Nitrogen Cycle01:49

The Nitrogen Cycle

60.4K
Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...
60.4K
Determining the pH of Salt Solutions04:08

Determining the pH of Salt Solutions

48.2K
The pH of a salt solution is determined by its component anions and cations. Salts that contain pH-neutral anions and the hydronium ion-producing cations form a solution with a pH less than 7. For example, in ammonium nitrate (NH4NO3) solution, NO3− ions do not react with water whereas NH4+ ions produce the hydronium ions resulting in the acidic solution.  In contrast, salts that contain pH-neutral cations and the hydroxide ion-producing anions form a solution with a pH greater than 7. For...
48.2K
Overview of Nitrogen Metabolism01:20

Overview of Nitrogen Metabolism

11.4K
Nitrogen is a very important element for life because it is a major constituent of proteins and nucleic acids. It is a macronutrient, and in nature, it is recycled from organic compounds and stored in the form of  ammonia, ammonium ions, nitrate, nitrite, or  nitrogen gas by many metabolic processes. Many of these metabolic processes are carried out only by prokaryotes.
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
11.4K
Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

553
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
553
Chirality at Nitrogen, Phosphorus, and Sulfur02:30

Chirality at Nitrogen, Phosphorus, and Sulfur

7.0K
Chirality is most prevalent in carbon-based tetrahedral compounds, but this important facet of molecular symmetry extends to sp3-hybridized nitrogen, phosphorus and sulfur centers, including trivalent molecules with lone pairs. Here, the lone pair behaves as a functional group in addition to the other three substituents to form an analogous tetrahedral center that can be chiral.
A consequence of chirality is the need for enantiomeric resolution. While this is theoretically possible for all...
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The OsMYB4 transcription factor enables coordinated improvement of grain yield and quality in rice.

Nature communications·2026
Same author

The ELD4-OsPRR95 module represses OsMADS51 in regulating rice heading date.

The Plant cell·2026
Same author

Redundant Glycerol-3-Phosphate Acyltransferases Regulate Thermo-Sensitive Genic Male Sterility in Rice.

Plant biotechnology journal·2026
Same author

Abnormal meiocytes and tapetum 1 (OsAMT1) is essential for proper meiosis and tapetal development in rice.

The Plant journal : for cell and molecular biology·2026
Same author

Natural variation in OsTPS8 confers differential regulation of chalkiness and seed vigor in indica and japonica rice.

Nature genetics·2025
Same author

Perturbation of Anion/Cation Transport Leads to Apical Panicle Abortion in Rice by Disrupting Ca<sup>2+</sup> Homeostasis.

Plant biotechnology journal·2025

Related Experiment Video

Updated: Feb 8, 2026

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.
08:27

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.

Published on: November 30, 2022

5.3K

An Elite Haplotype of Nitrogen-Use-Efficiency Gene LHT5 Enhances Salt Tolerance in Rice.

Saisai Wang1, Xingzhou Jiang1, Gaoming Chen1,2

  • 1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Jiangsu Zhongshan Biological Breeding Laboratory, Nanjing, China.

Plant Biotechnology Journal
|February 7, 2026
PubMed
Summary

Researchers identified a rice gene, LHT5, that improves nitrogen use efficiency (NUE) and salt tolerance by regulating amino acid transport. An elite LHT5 variant enhances these traits, offering potential for climate-resilient crop development.

Keywords:
Oryza sativa L.lysine‐histidine‐type transporternitrogen use efficiencysalt tolerancesuperior allele

More Related Videos

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions
07:43

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions

Published on: March 14, 2025

825
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.5K

Related Experiment Videos

Last Updated: Feb 8, 2026

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.
08:27

Analysis of Effect of Compound Salt Stress on Seed Germination and Salt Tolerance Analysis of Pepper Capsicum annuum L.

Published on: November 30, 2022

5.3K
Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions
07:43

Production of Arbuscular Mycorrhizal (AM) Fungal Inoculum and Phenotypic Evaluation of Rice and AM Symbiosis Under Saline Conditions

Published on: March 14, 2025

825
Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
09:43

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

3.5K

Area of Science:

  • Plant Biology
  • Genetics
  • Agricultural Science

Background:

  • Amino acids are crucial for plant stress adaptation and nutrient metabolism.
  • Coordinating nitrogen use efficiency (NUE) and salt tolerance in plants is a significant challenge.

Purpose of the Study:

  • To identify genes regulating nutrient metabolism for enhanced NUE and salt tolerance in rice.
  • To elucidate the molecular mechanism linking amino acid transport to NUE and salt tolerance.

Main Methods:

  • Genome-wide association studies (GWAS) to identify candidate genes.
  • Functional validation of the LHT5 gene and its elite haplotype (LHT5^HapA).
  • Analysis of amino acid accumulation, proline biosynthesis, and physiological stress responses.

Main Results:

  • A Lysine-Histidine-type transporter 5 (LHT5) gene was identified, enhancing NUE via amino acid accumulation.
  • OsLHT5 confers salt tolerance by upregulating proline biosynthesis genes (OsP5CS1, OsP5CS2).
  • An elite haplotype (LHT5^HapA) with a 30-bp deletion significantly boosts amino acid transport, improving NUE, salt tolerance, and grain yield.

Conclusions:

  • LHT5 plays a key role in coordinating NUE and salt tolerance through amino acid transport.
  • The elite LHT5^HapA haplotype offers a valuable genetic resource for developing climate-resilient rice.
  • This study provides a novel molecular framework for improving crop productivity under environmental stress.