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Updated: Feb 8, 2026

Rescue of Recombinant Zika Virus from a Bacterial Artificial Chromosome cDNA Clone
Published on: June 24, 2019
Generation of an infectious cDNA clone of BJ-Swzp-2022, a Group III Isolate of Getah Virus
Dongni Kong1, Liang Meng2, Dan Liu1
1China Institute of Veterinary Drug Control, No. 8 Nandajie, Zhongguancun, Haidian, Beijing, 100081, China.
Abstract:
Getah virus (GETV), a mosquito-borne arbovirus, possesses many susceptible hosts, including pigs, horses, cattle, and blue foxes. Currently, the biological characteristics and pathogenic mechanisms of GETV remain to be investigated. A GETV, isolated from a contaminated Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) live vaccine, was designated as BJ-Swzp-2022. Subsequently, the complete genome of the GETV BJ-Swzp-2022 was sequenced to construct a full-length infectious cDNA clone using T7 RNA polymerase. Transfection of BSR T7/5 cells expressing T7 RNA polymerase with this infectious clone resulted in the rescue of GETV, which exhibited replication and replication characteristics similar to those of the parental virus. Establishing this platform would facilitate understanding the pathogenic mechanisms of GETV and developing novel vaccines.
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