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Related Experiment Video

Updated: Feb 9, 2026

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Deterministic Evolution of Aptamers via a Microfluidic-Integrated Robotic Platform Using Complex Exosomes as Targets.

Binyao Liu1,2, Yaning Liu3, Lei Qiu3

  • 1School of Biomedical Engineering, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui 230026, P.R. China.

ACS Nano
|February 7, 2026
PubMed
Summary

Researchers developed DREAMbot, an automated system for discovering aptamers that bind to exosomes (EXOs). This robotic platform enhances exosome biomarker discovery for liquid biopsies by overcoming separation challenges in aptamer selection.

Keywords:
DLD sortingaptamer SELEXexosomemicrofluidic automationrobot pipetting

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Area of Science:

  • Biotechnology
  • Biomarker Discovery
  • Molecular Diagnostics

Background:

  • Exosome (EXO) membrane proteins are valuable biomarkers for liquid biopsy.
  • Antibody-based reagents face challenges due to exosome heterogeneity.
  • Traditional aptamer selection (SELEX) is complicated by the small size and separation difficulties of exosomes.

Purpose of the Study:

  • To develop an automated system for efficient exosome-targeted aptamer selection.
  • To overcome the limitations of manual SELEX for nanosized exosomes.
  • To create a practical framework for aptamer discovery in liquid biopsy applications.

Main Methods:

  • Introduction of DREAMbot: a robotic platform integrating microfluidics for automated aptamer selection.
  • Utilized deterministic lateral displacement sorting and lipid-assisted magnetic isolation for exosome purification.
  • Executed multiround aptamer selection with minimal human intervention.

Main Results:

  • DREAMbot automated the selection and recovery of exosome-binding aptamers.
  • The system demonstrated faithful aptamer enrichment, reducing hands-on time compared to manual SELEX.
  • Identified aptamers with nanomolar dissociation constants and high specificity for GPC3-positive exosomes from cell cultures and human serum.

Conclusions:

  • DREAMbot provides an automated, efficient, and accessible solution for aptamer discovery against exosomes.
  • The robotic-microfluidic platform facilitates biomarker identification for liquid biopsy.
  • This technology addresses key challenges in exosome-based diagnostics.