Correlating topography and viscoelastic properties of elastin-like polypeptide scaffolds probed at the nanoscale:...

The synthesis and property characterization of soft biomaterials has taken precedence in recent years. Although bulk physical-chemical properties are well known for these bio-materials, nanoscale properties still need to be probed and evaluated to fine tune the bio-compatibility (structural as well as functional) with natural tissues for regenerative medicine, prosthetics and other biological applications. In this study, the focus is on a popular soft biomaterial, Elastin-like polypeptide (ELP) which has been prepared under different pH conditions. The topographical features of the ELP at the nanoscale using Atomic Force Microscopy (AFM) are explored. Additionally, the employment of a non linear mode of AFM called Intermodulation-AFM (ImAFM) to correlate the elastic properties (Young's modulus) of ELP probed at the nanoscale with the topographical features gives us a deep insight into the mechanical properties offered by ELP when the structural features are altered by change in the ELP synthesis conditions namely, pH in this study. The noteworthy point is that these properties are measured at a spatial resolution of 0.9 nm. Finally, the change in the structural features of ELP with varying pH is discussed through atomistic Molecular Dynamics Simulations. The interaction mechanisms of the amino acid sequences and crosslinkers with proteins as they form the backbone and sidechain of the ELP at different pH are explored.