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Updated: Feb 9, 2026

DNA-affinity-purified Chip DAP-chip Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
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Refactoring two-component systems for tunable gene expression regulation and upgraded bacterial sensing.

Sheng-Yan Chen1, Haoran Xu2, Xinyi Wan3

  • 1Key Laboratory of Biomass Chemical Engineering of Ministry of Education, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310058, China; ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou 311215, China; School of Chemistry and Chemical Engineering, State Key Laboratory Incubation Base for Green Processing of Chemical Engineering, Shihezi University, Shihezi 832003, China.

Cell Systems
|February 7, 2026
PubMed
Summary

This study refactors two-component systems (TCSs), revealing response regulators (RRs) and histidine kinases (HKs) as key for programmability. Engineered TCS sensors offer ultrasensitive detection and improved biosensor performance.

Keywords:
gene expression regulationgenetically encoded biosensorlow noise amplifierswitch-like circuitsynergistic sensing systemtwo-component system

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