Related Experiment Video

Updated: Feb 9, 2026

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence
06:25

Quantifying Replication Stress in Ovarian Cancer Cells Using Single-Stranded DNA Immunofluorescence

Published on: February 10, 2023

2.5K

FSHR-targeting tetrahedral DNA frameworks ameliorate ovarian aging through oxidative stress elimination

Yun Dai1,2,3, Yican Guo1,2,3, Dan Chen1,2,3

  • 1Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430030, Hubei, China.

Journal of Nanobiotechnology
|February 7, 2026
PubMed

Insights

This study introduces FSH-DTN, a novel nanoparticle that targets the ovaries to combat oxidative stress and delay ovarian aging. It demonstrates superior antioxidant and anti-aging effects, offering a new strategy for preserving ovarian function.

Area of Science:

  • Reproductive biology
  • Gerontology
  • Nanomedicine

Background:

  • Ovarian aging, marked by reduced ovarian reserve, accelerates female aging.
  • Oxidative stress drives ovarian aging through apoptosis, mitochondrial dysfunction, and inflammation.
  • Existing antioxidants lack proven efficacy and have side effects, necessitating novel solutions.

Purpose of the Study:

  • To develop and evaluate FSH-DTN, a targeted nanoparticle for ovarian delivery.
  • To assess FSH-DTN's efficacy in mitigating oxidative stress and delaying ovarian aging.
  • To elucidate the mechanisms underlying FSH-DTN's protective effects on ovarian function.

Main Methods:

  • Development of FSH-DTN, a DNA tetrahedral nanostructure with a follicle-stimulating hormone receptor-targeting peptide.
  • In vivo imaging to confirm ovarian targeting efficiency of FSH-DTN.
  • In vitro and in vivo models of acute, subacute, and chronic ovarian aging to evaluate FSH-DTN's effects.
  • Analysis of ROS scavenging, apoptosis, NRF2 pathway activation, and ovarian function.

Main Results:

  • FSH-DTN exhibited enhanced ovarian accumulation compared to native DTN.
  • FSH-DTN demonstrated significant antioxidant, anti-apoptotic, and anti-aging effects in ovarian aging models.
  • FSH-DTN directly scavenged reactive oxygen species (ROS) and activated the NRF2 pathway, boosting endogenous antioxidant capacity.
  • Ovarian function was protected by FSH-DTN treatment.

Conclusions:

  • FSH-DTN is a promising nanotherapeutic for targeting ovarian aging.
  • This targeted nanoparticle effectively neutralizes ovarian oxidative stress.
  • FSH-DTN represents a novel strategy to delay ovarian aging and preserve reproductive health.
Keywords:
DNA nanotechnologyFollicle-stimulating hormone receptorFramework nucleic acidOvarian agingOxidative stress

More Related Videos

Comet Assay as an Indirect Measure of Systemic Oxidative Stress08:23

Comet Assay as an Indirect Measure of Systemic Oxidative Stress

Published on: May 22, 2015

29.9K
Analysis of Oxidative Stress in Zebrafish Embryos11:05

Analysis of Oxidative Stress in Zebrafish Embryos

Published on: July 7, 2014

38.2K

Related Concept Videos

Crystal Field Theory - Tetrahedral and Square Planar Complexes02:46

Crystal Field Theory - Tetrahedral and Square Planar Complexes

Tetrahedral Complexes
Crystal field theory (CFT) is applicable to molecules in geometries other than octahedral. In octahedral complexes, the lobes of the dx2−y2 and dz2 orbitals point directly at the ligands. For tetrahedral complexes, the d orbitals remain in place, but with only four ligands located between the axes. None of the orbitals points directly at the tetrahedral ligands. However, the dx2−y2 and dz2 orbitals (along the Cartesian axes) overlap with the ligands less than the dxy,...
48.6K
Oxidation Numbers03:14

Oxidation Numbers

In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.9K
Pyruvate Oxidation01:15

Pyruvate Oxidation

After glycolysis, the charged pyruvate molecules enter the mitochondria via active transport and undergo three enzymatic reactions. These reactions ensure that pyruvate can enter the next metabolic pathway so that energy stored in the pyruvate molecules can be harnessed by the cells.
First, the enzyme pyruvate dehydrogenase removes the carboxyl group from pyruvate and releases it as carbon dioxide. The stripped molecule is then oxidized and releases electrons, which are then picked up by NAD+...
169.1K
Aging01:26

Aging

Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
Cellular Clock Theory
The cellular clock theory posits that the human lifespan is closely tied to the finite capacity of cells to divide, a phenomenon governed by telomeres, which are protective caps at the ends of...
719
DNA Topoisomerases02:02

DNA Topoisomerases

Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
35.7K
Oxidation-Reduction Reactions03:11

Oxidation-Reduction Reactions

Oxidation–Reduction Reactions
75.8K