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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Principles Of Column Chromatography01:13

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The chromatography technique was first invented in 1901 by Michael S. Tswett, a Russian botanist, to separate plant pigments using organic solvents. Further, in 1941, Archer John Porter Martin and R. L. M. Synge modified the technique by packing silica gel into a column. A mixture of amino acids was then separated on the packed column using chloroform and water mixture as the mobile phase. This was the first report on column chromatography. At present, column chromatography is a widely used...
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Bernoulli's Principle: Applications01:17

Bernoulli's Principle: Applications

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There are many devices and situations in which fluid flows at a constant height and so can be analyzed using Bernoulli's principle. These devices include, but are not limited to, entrainment devices and fluid flow measuring devices.
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Design Example: Application of Archimedes' Principle01:11

Design Example: Application of Archimedes' Principle

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Archimedes' principle is fundamental in analyzing the buoyant force and stability of floating bodies. In this example, a wooden block with a rectangular section floats in seawater. Based on the block's dimensions, its specific gravity and the specific weight of seawater are used to find the volume of water displaced and the center of buoyancy.
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Electron Affinity03:07

Electron Affinity

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The electron affinity (EA) is the energy change for adding an electron to a gaseous atom to form an anion (negative ion).
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Affinity and Avidity01:41

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Protein Purification-free Method of Binding Affinity Determination by Microscale Thermophoresis
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Microscale Affinity Chromatography for Biointeraction Analysis: Strategies, Principles and Applications.

David S Hage1, Nigar Sultana Pinky1, B K Sajeeb1

  • 1Department of Chemistry, University of Nebraska-Lincoln, Lincoln, Nebraska, USA.

Journal of Separation Science
|February 8, 2026
PubMed
Summary
This summary is machine-generated.

Microscale affinity chromatography (µAC) offers powerful tools for studying biointeractions in biochemical and biomedical research. This review covers µAC platforms and strategies for analyzing molecular interactions and kinetics.

Keywords:
affinity microcolumnbinding studiesbiointeraction analysiskinetic studiesmicroscale affinity chromatography

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Area of Science:

  • Biochemistry
  • Biomedical Research
  • Environmental Science

Background:

  • Analyzing interactions between biological agents and chemicals is crucial for modern research.
  • Microscale platforms utilizing affinity chromatography (µAC) are effective tools for these analyses.
  • µAC employs immobilized binding agents for direct interaction studies or as capture agents.

Purpose of the Study:

  • To review various platforms and strategies used in microscale affinity chromatography (µAC).
  • To examine the principles, schemes, and applications of µAC in characterizing biointeractions.
  • To provide examples of µAC methods, including zonal and frontal analysis, and kinetic studies.

Main Methods:

  • Review of existing literature on microscale affinity chromatography platforms and strategies.
  • Examination of general principles and schemes of µAC.
  • Analysis of applications in biointeraction studies, including kinetic studies.

Main Results:

  • Microscale affinity chromatography provides versatile platforms for studying biointeractions.
  • Various analytical methods like zonal and frontal analysis are applicable within µAC.
  • µAC is effective for both direct interaction analysis and probing solution-phase interactions.

Conclusions:

  • Microscale affinity chromatography is a powerful technique for characterizing biointeractions.
  • The principles and applications of µAC, including kinetic studies, are well-established.
  • µAC platforms offer significant advantages in biochemical, biomedical, and environmental research.