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Updated: Feb 10, 2026

Exploring the Arginine Methylome by Nuclear Magnetic Resonance Spectroscopy
Published on: December 16, 2021
Spectral-band-selective multidimensional nuclear magnetic resonance spectroscopy using broadband dipolar recoupling
Edward P Saliba1, Sarah A Overall2, Alexander B Barnes2
1Swiss Federal Institute of Technology in Zürich, Institute for Molecular Physical Science, HCI, Vladimir-Prelog-Weg 2, 8093 Zürich, Switzerland; Ohio University, Department of Chemistry and Biochemistry, 133 University Terrace, Athens, OH 45701, USA.
Abstract:
Nuclear magnetic resonance (NMR) spectroscopy is an important analytical tool for probing molecular structures and interactions. For high complexity samples, multidimensional spectroscopy is essential for improving the resolution of NMR data. However, multidimensional experiments cost significant experimental time which scales with the number of indirect points. This is particularly challenging when dealing with highly dispersed nuclei, such as 13C, due to the large chemical shift range, with large regions that are spectrally empty. Herein, we describe a method for limiting the spectral width of dipolar based multidimensional NMR experiments in the indirect dimension in a manner that can be easily integrated into relaxation and distance measuring schemes. We demonstrate the acquisition of narrow strips of broadband homonuclear recoupling 13C-13C correlation spectra on a range of biomolecular and cellular samples, allowing targeted acquisition of high-resolution spectra of the region of interest with a significant reduction in the acquisition time. We also demonstrate the use of the spectral-band-selective method for allowing fast acquisition of RFDR build-up experiments. The band-selective method is easy to implement in any dipolar-based multidimensional pulse sequence by an addition of one pulse per band-selected indirect dimension and a slight modification of the phase cycle.
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