Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chemical Reactions in Aqueous Solutions03:03

Chemical Reactions in Aqueous Solutions

72.6K
Chemical substances interact in many different ways. Certain chemical reactions exhibit common patterns of reactivity. Due to the vast number of chemical reactions, it becomes necessary to classify them based on the observed patterns of interaction.
72.6K
Aqueous Solutions and Heats of Hydration02:42

Aqueous Solutions and Heats of Hydration

18.0K
Water and other polar molecules are attracted to ions. The electrostatic attraction between an ion and a molecule with a dipole is called an ion-dipole attraction. These attractions play an important role in the dissolution of ionic compounds in water.
When ionic compounds dissolve in water, the ions in the solid separate and disperse uniformly throughout the solution because water molecules surround and solvate the ions, reducing the strong electrostatic forces between them. This process...
18.0K
Leveling Effect and Non-Aqueous Acid-Base Solutions02:11

Leveling Effect and Non-Aqueous Acid-Base Solutions

9.7K
This lesson defines the leveling effect in acidic and basic solutions and its role in aqueous and non-aqueous solutions. It is essential to understand the competing nature of various species in a chemical system.
The Leveling Effect of a Solvent
A generic acid (HA) reacts with the generic base (B-) to yield the corresponding conjugate base (A-) and conjugate acid (HB):
9.7K
Introduction to Membrane Proteins01:16

Introduction to Membrane Proteins

81.4K
The cell membrane, or plasma membrane, is an ever-changing landscape. It is described as a fluid mosaic where various macromolecules are embedded in the phospholipid bilayer. Among the macromolecules are proteins. The protein content varies across cell types. For example, mitochondrial inner membranes contain ~76% protein content, while myelin contains ~18% protein content. Individual cells contain many types of membrane proteins—red blood cells contain over 50—and different cell...
81.4K
Membrane Proteins01:30

Membrane Proteins

30.5K
Plasma membranes have integral transmembrane proteins involved in facilitated transport. These proteins are collectively referred to as transport proteins, and they function as either channels for the material or as carriers themselves. Channel proteins have hydrophilic domains exposed to the intracellular and extracellular fluids and a hydrophilic channel through their core that provides a hydrated opening for solutes to pass through the membrane layers. Passage through the channel allows...
30.5K
General Properties of Solutions02:12

General Properties of Solutions

36.0K
Many common substances around us exist as a solution, such as ocean water, air, and gasoline. All solutions are mixtures of substances that are composed of varying amounts of two or more types of atoms or molecules. A mixture with a non-uniform composition is a heterogeneous mixture, whereas a mixture with a uniform composition is a homogeneous mixture. The components that make the homogeneous mixture are evenly spread out and thoroughly mixed. 
36.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PepMCP: A Graph-Based Membrane Contact Probability Predictor for Membrane-Lytic Antimicrobial Peptides.

Bioinformatics (Oxford, England)·2026
Same author

Cryo-EM structures of heteromeric Kir4.1/5.1 channel suggest mechanisms of inward rectification and channel blockage.

Nature communications·2026
Same author

Navigating high-order protein fitness landscapes via deep learning on directed evolution trajectories.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Electroporation-based death of bacteria on assembled piezoelectric nanoenzyme-tips for implant disinfection.

Biomaterials·2026
Same author

Optimization of RNP-CRISPR for high-efficiency gene editing in mouse intestinal organoids.

Molecular and cellular biochemistry·2026
Same author

ProtRAP-LM: Efficient Protein Relative Accessibility Prediction and Proteome-wide Membrane Protein Screening.

Genomics, proteomics & bioinformatics·2026
Same journal

Continuous Information Descriptors for Electron Localization: Relativistic Spatial Responses, Nonadditivity, and Chemical Bonding.

Journal of chemical theory and computation·2026
Same journal

Determining Quantum Mechanical Methods Suitable for Quantitative Modeling of Hydrogen Atom Transfer by Halogen Atoms.

Journal of chemical theory and computation·2026
Same journal

Predicting Solvation Free Energies of Molecules and Ions via First-Principles and Machine-Learning Molecular Dynamics.

Journal of chemical theory and computation·2026
Same journal

Connection between <i>GW</i> and Extended Coupled Cluster.

Journal of chemical theory and computation·2026
Same journal

Resolving Local and Global Conformational Heterogeneity of the Human Intrinsically Disordered Proteome.

Journal of chemical theory and computation·2026
Same journal

Molecular Modeling of Surfactant Interaction on Phospholipid Bilayers Mimicking Corneal Epithelium.

Journal of chemical theory and computation·2026
See all related articles

Related Experiment Video

Updated: Feb 10, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K

MemConverter: An Iterative Pipeline for Reprogramming Protein Localization in Membrane or Aqueous Solution.

Jun Li1,2,3, Haozhe Guo1,2, Chen Song1,2

  • 1Center for Quantitative Biology, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.

Journal of Chemical Theory and Computation
|February 9, 2026
PubMed
Summary
This summary is machine-generated.

We developed MemConverter, a computational pipeline that converts soluble proteins into membrane proteins and vice versa. This tool uses membrane contact probability (MCP) to engineer protein localization for enhanced solubility or membrane integration.

More Related Videos

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

9.8K
The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
11:45

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

10.2K

Related Experiment Videos

Last Updated: Feb 10, 2026

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

33.6K
Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

9.8K
The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
11:45

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Published on: February 27, 2020

10.2K

Area of Science:

  • Computational biology
  • Protein engineering
  • Biophysics

Background:

  • Engineering proteins for specific cellular localization is crucial for biotechnology and therapeutics.
  • Existing methods for protein localization reprogramming often lack precision and efficiency.
  • Predicting and controlling protein-environment interactions, particularly membrane association, remains a challenge.

Purpose of the Study:

  • To introduce MemConverter, a novel computational pipeline for converting soluble proteins to membrane proteins and vice versa.
  • To enable precise reprogramming of protein surface properties for targeted localization.
  • To provide a validated computational tool for designing proteins for membrane or aqueous environments.

Main Methods:

  • Development of MemProtMPNN by fine-tuning ProteinMPNN on a membrane protein dataset.
  • Integration of MemProtMPNN with AlphaFold2 for iterative structure refinement.
  • Utilization of membrane contact probability (MCP) for guided sequence fusion and protein design.
  • Validation using molecular dynamics simulations.

Main Results:

  • The MemConverter pipeline demonstrated superior performance over existing methods.
  • Designed proteins showed stable membrane integration or enhanced solubility, as confirmed by molecular dynamics simulations.
  • Successful reprogramming of protein localization based on MCP predictions.

Conclusions:

  • MemConverter offers a powerful new computational approach for engineering protein localization.
  • The pipeline facilitates the design of proteins for targeted integration into membranes or soluble environments.
  • This work advances the field of protein engineering with a tool for precise control over protein localization.