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A novel MYB transcription factor, BnaC04.MYB100, regulates flowering time in rapeseed by activating gibberellin (GA) biosynthesis. This discovery offers a new target for improving crop yield and adaptation.

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Area of Science:

  • Plant Biology
  • Genetics
  • Agricultural Science

Background:

  • Flowering time in Brassica napus (rapeseed) is crucial for crop adaptation and yield.
  • Molecular mechanisms connecting MYB transcription factors, gibberellin (GA) biosynthesis, and flowering control in rapeseed remain incompletely understood.

Purpose of the Study:

  • To identify and characterize MYB transcription factors involved in regulating flowering time and GA biosynthesis in rapeseed.
  • To elucidate the molecular link between BnaC04.MYB100, GA pathway, and flowering time.

Main Methods:

  • Gene cloning and functional analysis (overexpression and CRISPR/Cas9 knockout).
  • Transcriptome and hormone analyses to assess gene expression and GA levels.
  • Molecular assays including dual-luciferase reporter, yeast one-hybrid, ChIP-qPCR, and EMSA to confirm direct binding.
  • Analysis of natural variation and artificial selection in rapeseed germplasm.

Main Results:

  • BnaC04.MYB100 acts as a positive regulator of flowering time; its overexpression accelerates flowering, while knockout delays it.
  • BnaC04.MYB100 upregulates flowering-time genes and GA biosynthesis, increasing endogenous GA levels.
  • BnaC04.MYB100 directly binds to the promoter of BnaGA20ox3, a key GA biosynthetic gene.
  • Natural variation in BnaC04.MYB100 is linked to flowering time diversity and shows signs of artificial selection.

Conclusions:

  • BnaC04.MYB100 is a key integrator connecting GA biosynthesis with flowering time control in rapeseed.
  • This transcription factor represents a valuable genetic target for enhancing rapeseed breeding for optimized flowering time and yield.