Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.2K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.2K
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

7.3K
Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
7.3K
Appendicitis-II: Diagnostic Studies and Management01:29

Appendicitis-II: Diagnostic Studies and Management

589
Diagnosing and managing appendicitis requires a structured and comprehensive approach that spans from initial assessment to postoperative care. Here is an overview of the process:
Diagnosing Appendicitis
It requires a multifaceted approach, starting with a detailed physical examination to pinpoint the location and nature of the pain and identify any associated symptoms. Laboratory tests play a crucial role. A complete Blood Count (CBC) typically reveals leukocytosis (an increased number of...
589
Myasthenia Gravis: Diagnostic Tests01:15

Myasthenia Gravis: Diagnostic Tests

2.7K
Myasthenia gravis is an autoimmune condition affecting neuromuscular transmission, causing generalized weakness in skeletal muscles. Initial diagnoses rely on patients' signs, symptoms, and medical history. The challenge lies in distinguishing myasthenia from other muscular dystrophies. An important diagnostic feature is the significant improvement of symptoms after administering anticholinesterase inhibitors.
The edrophonium test is a diagnostic tool for myasthenia gravis. It involves...
2.7K
Asthma-IV: Diagnostic and Management01:30

Asthma-IV: Diagnostic and Management

3.2K
The diagnosis and management of asthma are comprehensive, encompassing clinical assessments, lung function tests, and pharmacological interventions. Here's an overview:
Clinical Assessment for Asthma:
This is the first step in diagnosing and managing asthma. It includes:
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Oxidative stress-induced astrocytic collagen biosynthesis drives glial barrier formation and neuronal death in ischemic stroke.

Cell metabolism·2026
Same author

Cannabidiol modulates spontaneous recovery through distinct and conserved transcriptomic signatures in mPFC subregions.

Communications biology·2026
Same author

AI-Based Respiratory Monitoring-Guided Evaluation of Rottlerin Therapy for PRRS in Grower-Finisher Pig Farms.

Viruses·2026
Same author

metaFun: An analysis pipeline for metagenomic big data with fast and unified functional searches.

Gut microbes·2026
Same author

Translational value of understanding brain-spinal interactions in persistent pain.

Neural regeneration research·2025
Same author

Polyphasic and comparative genomic characterization of a novel Mariniflexile species in the rhizosphere microbiome of tomato resistant to bacterial wilt.

Scientific reports·2025

Related Experiment Video

Updated: Feb 10, 2026

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.2K

A CRISPR-Cas13a-Based Amplification- and Extraction-Free Fire Blight Diagnostic System.

Ye Ram Cho1, Boyoung Lee1, Chang-Sik Oh2

  • 1Department of Systems Biology, Division of Life Sciences, and Institute for Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.

The Plant Pathology Journal
|February 9, 2026
PubMed
Summary
This summary is machine-generated.

A new CRISPR-Cas13a diagnostic tool enables rapid, extraction-free detection of fire blight (Erwinia amylovora) directly from plants. This innovation improves disease management in apple and pear orchards by overcoming limitations of traditional methods.

Keywords:
biomarkerdiagnosisdirect detectionpoint-of-carequarantine pathogen

More Related Videos

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.9K
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

3.2K

Related Experiment Videos

Last Updated: Feb 10, 2026

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

2.2K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.9K
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

3.2K

Area of Science:

  • Plant Pathology
  • Molecular Diagnostics
  • Bacteriology

Background:

  • Fire blight, caused by Erwinia amylovora, is a major economic threat to apple and pear production.
  • Current diagnostic methods are slow, require nucleic acid extraction, and struggle with plant-derived inhibitors, hindering rapid field detection.
  • Distinguishing E. amylovora from similar species like E. pyrifoliae is crucial for accurate disease management.

Purpose of the Study:

  • To develop a rapid, amplification-free, and nucleic acid extraction-free diagnostic platform for fire blight using CRISPR-Cas13a.
  • To design species-specific CRISPR RNAs (crRNAs) for accurate detection of Erwinia amylovora.
  • To establish a field-compatible sample processing method for direct plant-to-diagnostic testing.

Main Methods:

  • Identification of E. amylovora-specific single nucleotide polymorphisms (SNPs).
  • Design and screening of multiple crRNAs targeting housekeeping genes and 16S rRNA V3 region.
  • Development of an alkaline lysis workflow for direct RNA release from plant material compatible with CRISPR-Cas13a detection.

Main Results:

  • Identified sensitive and specific crRNAs for rapid detection of E. amylovora within minutes.
  • Demonstrated successful extraction-free detection of E. amylovora directly from crude apple leaf lysates.
  • Achieved detection of 1 × 10^6 Colony Forming Units (CFUs) per reaction in 15 minutes without nucleic acid purification or thermal cycling.

Conclusions:

  • The CRISPR-Cas13a platform offers a rapid, sensitive, and specific method for fire blight detection directly from plant samples.
  • The developed alkaline lysis workflow enables field-deployable, low-infrastructure diagnostics.
  • This approach significantly enhances the speed and accuracy of fire blight surveillance and agricultural biosecurity.