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Updated: Feb 10, 2026

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
Published on: March 17, 2023
A coumarin functionalized NIR fluorescent probe based on the thiopyrone skeleton for the detection of Cys and its
Huan Zhang1, Baoze Guo1, Junqing Zhou1
1Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, School of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, P. R. China. youlai@tjut.edu.cn.
Abstract:
Cysteine fluorescent probes are specialized molecular tools that facilitate highly sensitive detection of cysteine via alterations in fluorescent signals. Currently, these probes have been widely employed in fields including disease biomarker monitoring, redox balance research, and drug toxicity assessment, thereby exhibiting substantial application potential in biochemical and biomedical studies. In this study, a novel fluorescent probe was designed for detecting cysteine based on the thiopyrone structure. Through characterization of its properties, it was found that this fluorescent probe exhibits a large Stokes shift (217 nm), excellent sensitivity (13.60 nM), rapid response time (3.0 min), high stability and selectivity. Furthermore, this cysteine fluorescent probe demonstrates excellent applications in RAW 264.7 cells, zebrafish, and actual samples. This study also proposes a more convenient method for testing cysteine levels using mobile phone software, and the findings indicate that the fluorescent probe under investigation has considerable potential for use in cysteine detection.
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