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Related Experiment Video

Updated: Feb 10, 2026

Permeabilization of Adhered Cells Using an Inert Gas Jet
08:21

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Published on: September 4, 2013

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Open source inert gas glove box.

Mottaghi Maryam1, M Pearce Joshua2

  • 1Department of Mechanical and Materials Engineering, Western University, London, Canada.

Hardwarex
|February 9, 2026
PubMed
Summary
This summary is machine-generated.

An open-source glove box was developed using 3D printing, offering a low-cost, customizable, and portable solution for scientific research. This validated design significantly reduces oxygen levels, making controlled environments more accessible for under-resourced laboratories.

Keywords:
3D printingEnvironment safetyEnvironmental controlGlove boxInert gasOpen hardware

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Area of Science:

  • Materials Science
  • Engineering
  • Laboratory Equipment

Background:

  • Glove boxes are essential controlled environments for scientific experiments.
  • High costs of commercial glove boxes ($1,000-$15,000+) limit accessibility in under-resourced labs.
  • Existing low-cost DIY designs lack validation and characterization.

Purpose of the Study:

  • To develop an open-source glove box design for scientific applications.
  • To overcome cost and accessibility limitations of commercial glove boxes.
  • To validate the performance and cost-effectiveness of the open-source design.

Main Methods:

  • Utilized readily available components and digital distributed manufacturing (open-source 3D printers).
  • Quantified inert atmosphere capability using an oxygen analyzer.
  • Designed for customizable dimensions and transfer chambers.

Main Results:

  • Achieved significant oxygen reduction down to 19 ppm using an inert gas.
  • Demonstrated over 95% economic savings compared to proprietary systems.
  • The design is portable, customizable, and prevents contamination.

Conclusions:

  • The developed open-source glove box is a validated, low-cost, and effective alternative to commercial systems.
  • This design enhances accessibility of controlled environments for scientific research.
  • Customizable features and portability make it suitable for diverse applications.