Native yeast kinetochore structures identify an essential inner kinetochore interaction
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View abstract on PubMed
Summary
This summary is machine-generated.The Ndc10 protein
Area Of Science
- Cell Biology
- Molecular Biology
- Structural Biology
Background
- Accurate kinetochore assembly on centromeres is crucial for cell division.
- Budding yeast centromeric DNA is challenging for nucleosome formation due to bending resistance.
Purpose Of The Study
- To investigate the assembly mechanism of the yeast inner kinetochore.
- To understand the role of Ndc10 in centromere structure and kinetochore formation.
Main Methods
- Developed a one-step protocol for purifying de novo assembled native inner kinetochore subcomplexes.
- Utilized cryoelectron microscopy (cryo-EM) for structural analysis of purified complexes.
Main Results
- Generated medium to high-resolution cryo-EM density maps for four inner kinetochore complexes, including two previously unvisualized.
- Identified an Ndc10 trimerization domain that binds centromeric DNA and CBF3 complexes, inducing significant centromere bending.
- Observed differences between native and reconstituted structures, indicating de novo assembly captured intermediate states.
Conclusions
- Ndc10 trimerization is essential for bending centromeric DNA, facilitating nucleosome and inner kinetochore assembly and stabilization.
- This mechanism is vital for proper kinetochore function and faithful chromosome segregation.
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