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Updated: Feb 10, 2026

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Native yeast kinetochore structures identify an essential inner kinetochore interaction.

Mengqiu Jiang1, Changkun Hu1, Sabrine Hedouin2

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Summary

The Ndc10 protein

Keywords:
CentromereChromosome segregationDNA bendingKinetochoreNdc10

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Structural Biology

Background:

  • Accurate kinetochore assembly on centromeres is crucial for cell division.
  • Budding yeast centromeric DNA is challenging for nucleosome formation due to bending resistance.

Purpose of the Study:

  • To investigate the assembly mechanism of the yeast inner kinetochore.
  • To understand the role of Ndc10 in centromere structure and kinetochore formation.

Main Methods:

  • Developed a one-step protocol for purifying de novo assembled native inner kinetochore subcomplexes.
  • Utilized cryoelectron microscopy (cryo-EM) for structural analysis of purified complexes.

Main Results:

  • Generated medium to high-resolution cryo-EM density maps for four inner kinetochore complexes, including two previously unvisualized.
  • Identified an Ndc10 trimerization domain that binds centromeric DNA and CBF3 complexes, inducing significant centromere bending.
  • Observed differences between native and reconstituted structures, indicating de novo assembly captured intermediate states.

Conclusions:

  • Ndc10 trimerization is essential for bending centromeric DNA, facilitating nucleosome and inner kinetochore assembly and stabilization.
  • This mechanism is vital for proper kinetochore function and faithful chromosome segregation.