Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conformity01:20

Conformity

48.2K
Conformity is the change in a person’s behavior to go along with the group, even if that person does not agree with the group.
48.2K
GPCR Desensitization01:12

GPCR Desensitization

8.2K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
8.2K
Intrinsically Disordered Proteins02:18

Intrinsically Disordered Proteins

19.6K
Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
19.6K
Conformations of Butane02:20

Conformations of Butane

18.2K
Unlike ethane and propane that have only two major conformations, butane has more than two conformers. The staggered form of butane in which the bulky methyl groups on the two carbons are placed on opposite sides, that is, at a dihedral angle of 180°, is the lowest energy, most stable form — called the anti conformer. This conformation is stabilized due to the absence of steric repulsion between the largely spaced out methyl groups. The other two staggered conformations are...
18.2K
Rigid Body Equilibrium Problems - I00:49

Rigid Body Equilibrium Problems - I

5.5K
A rigid body is said to be in static equilibrium when the net force and the net torque acting on the system is equal to zero. To solve for rigid body equilibrium problems, do the following steps.
5.5K
Rigid Body Equilibrium Problems - II01:21

Rigid Body Equilibrium Problems - II

8.1K
A rigid body is in static equilibrium when the net force and the net torque acting on the system are equal to zero.
Consider two children sitting on a seesaw, which has negligible mass. The first child has a mass (m1) of 26 kg and sits at point A, which is 1.6 meters (r1) from the pivot point B; the second child has a mass (m2) of 32 kg and sits at point C. How far from the pivot point B should the second child sit (r2) to balance the seesaw?
8.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacological and Transcriptomic Exploration of β2-Adrenergic Receptor-Gα15 Signaling in THP-1-Derived Macrophages.

bioRxiv : the preprint server for biology·2026
Same author

Structural basis of opioid receptor activation by PCP and ketamine.

Nature structural & molecular biology·2026
Same author

Protease-activated receptor 1 as an endogenous model of peptidergic Gαq-Gα12-biased G protein signaling.

Frontiers in molecular biosciences·2026
Same author

De novo design of miniproteins targeting GPCRs.

Nature·2026
Same author

keju: powerful and accurate inference in Massively Parallel Reporter Assays.

bioRxiv : the preprint server for biology·2026
Same author

Author Correction: A µ-opioid receptor superagonist analgesic with minimal adverse effects.

Nature·2026

Related Experiment Video

Updated: Feb 10, 2026

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
09:12

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Published on: September 10, 2016

10.1K

Designing Rigid Protein Fiducials to Visualize GPCR Conformational States.

Alina Vo1, Yuan-En Sun2, Justin G English2

  • 1Verna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Baylor College of Medicine, Houston, TX, USA.

Biorxiv : the Preprint Server for Biology
|February 9, 2026
PubMed
Summary
This summary is machine-generated.

This study introduces a novel method using generative protein design and deep learning to create fiducial markers for visualizing G-protein coupled receptors (GPCRs) in challenging states. This breakthrough enables detailed structural analysis of previously intractable GPCR conformations.

More Related Videos

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
09:19

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Published on: March 16, 2020

7.5K
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
04:37

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

Published on: June 29, 2021

3.2K

Related Experiment Videos

Last Updated: Feb 10, 2026

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
09:12

G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay

Published on: September 10, 2016

10.1K
Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization
09:19

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Published on: March 16, 2020

7.5K
High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States
04:37

High-Pressure NMR Experiments for Detecting Protein Low-Lying Conformational States

Published on: June 29, 2021

3.2K

Area of Science:

  • Structural biology
  • Biophysics
  • Computational biology

Background:

  • G-protein coupled receptors (GPCRs) are crucial cell surface receptors involved in numerous physiological processes.
  • Visualizing GPCR conformational changes induced by ligands, especially without signaling partners, is a significant challenge in structural biology.
  • Understanding these conformational dynamics is key to developing targeted therapeutics.

Purpose of the Study:

  • To develop a generalizable method for visualizing previously intractable structural states of GPCRs.
  • To enable high-throughput determination of GPCR structures, particularly inactive states.
  • To characterize ligand-induced conformational changes in GPCRs without signaling partners.

Main Methods:

  • Integration of generative protein design with deep-learning based conformational ensemble prediction.
  • Design and application of 'fiducial markers' for cryogenic electron microscopy (cryoEM).
  • High-throughput structural determination of four pharmaceutically relevant GPCRs and detailed analysis of the β2-adrenergic receptor.

Main Results:

  • Successful design of fiducial markers enabling cryoEM visualization of GPCRs at arbitrary fusion points.
  • High-throughput determination of inactive state structures for four GPCRs, resolving key pharmacological details.
  • Direct structural characterization of intracellular motif rearrangements in the β2-adrenergic receptor in the absence of G-protein.

Conclusions:

  • The developed framework provides a generalizable approach to access traditionally inaccessible structural states of small, dynamic proteins like GPCRs.
  • This method overcomes limitations in visualizing ligand-induced conformational changes, offering new insights into GPCR pharmacology.
  • The findings pave the way for advanced structural studies of dynamic protein systems.