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Related Concept Videos

Transcription01:10

Transcription

157.1K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
157.1K
Transcription01:17

Transcription

33.4K
Transcription is the synthesis of RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in correctly synthesizing messenger RNA (mRNA). Transcriptional regulation is responsible for the differentiation of different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds of RNA Molecules
In eukaryotes,...
33.4K
Transcription Factors02:16

Transcription Factors

82.8K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.8K
Eukaryotic Transcription Inhibitors01:52

Eukaryotic Transcription Inhibitors

11.0K
Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...
11.0K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.9K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.9K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K

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Related Experiment Video

Updated: Feb 10, 2026

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience
08:14

Comprehensive Analysis of Transcription Dynamics from Brain Samples Following Behavioral Experience

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Dynamic Supercoiling Sponsors Transcription Amplification by MYC.

Rajiv Kumar Jha1, Fedor Kouzine1, Bo Wang1

  • 1Gene Regulation Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute (NCI), Bethesda, MD 20892-1500, USA.

Biorxiv : the Preprint Server for Biology
|February 9, 2026
PubMed
Summary
This summary is machine-generated.

MYC amplifies gene expression by interacting with DNA supercoiling. Reduced topoisomerase I activity enhances MYC

Keywords:
DNA supercoilingMYCTopoisomeraseTranscription-Amplifier

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Area of Science:

  • Molecular Biology
  • Cancer Research
  • Genetics

Background:

  • MYC dysregulation is common in cancer, impacting gene expression.
  • MYC's role as a transcription factor, particularly in amplification, is not fully understood.
  • The coordination between MYC's transcriptional role and its stimulation of topoisomerases is unclear.

Purpose of the Study:

  • To develop a genetic tool to differentiate MYC's transcriptional activation from amplification.
  • To investigate the interplay between MYC-driven transcription amplification and DNA supercoiling.
  • To examine how DNA topology regulates MYC activity.

Main Methods:

  • Development of a novel genetic system to distinguish MYC's activation versus amplification.
  • Utilized biochemical and cellular assays to study transcription dynamics.
  • Investigated the effects of reducing Topoisomerase I (TOP1) activity via genetic knockdown and low-dose inhibition.

Main Results:

  • Reduced TOP1 activity potentiates MYC-driven transcription amplification.
  • Increased DNA supercoiling stabilizes pre-initiation complexes (PICs).
  • MYC facilitates the mobilization of these stabilized PICs, enhancing amplification.

Conclusions:

  • DNA supercoiling is a critical regulator of MYC-dependent transcription amplification.
  • MYC functions as a universal amplifier of active genes, influenced by DNA topology.
  • Findings illuminate a novel mechanism controlling MYC's role in gene expression and cancer.