Mitogen-Activated Protein Kinase-3 (MAPK3) Is the Main Target of Microsecond Pulsed Electric Field in Human
Ayad Bahadorimonfared1, Zahra Razzaghi2, Reza M Robati3
1Department of Health & Community Medicine, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Introduction:
Pulsed electric fields (PEFs) are applied to facilitate the transfer of various types of molecules, such as pharmaceuticals, into living cells. In the present study effect of microsecond PEF on human medulloblastoma cell line (D283Med cells) is investigated to find the crucial targeted genes.
Methods:
Gene expression profiles of the human medulloblastoma (D283) cell line in response to microsecond PEF versus control are extracted from the Gene Expression Omnibus (GEO) database. The samples are analyzed via the GEO2R program to find the significant differentially expressed genes (DEGs). The significant DEGs were assessed via protein-protein interaction (PPI) network using Cytoscape software and its applications.
Results:
Among 410 significant DEGs, 23 hubs and bottlenecks were determined. A total number of seven hub-bottlenecks including MAPK3, KIT, APP, RUNX2, CXCR4, LMNA, and BMP4 were pointed out as the crucial targets of microsecond PEF. MAPK3 and KIT were down-regulated while, APP, RUNX2, CXCR4, LMNA, and BMP4 were up-regulated.
Conclusion:
It can be concluded that, down-regulation of MAPK3 and KIT and up-regulation of BMP4 which are the consequence of microsecond PEF treatment inhibit medulloblastoma. The results exhibited the significant role of MAPK3 in response to microsecond PEF treatment.
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