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[Interactions between nuclear polyhedrosis viruses and vertebrate cells (author's transl)]
Zentralblatt Fur Bakteriologie, Parasitenkunde, Infektionskrankheiten Und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie Und Parasitologie
|December 1, 1977
Summary
This study found no evidence of harm from Autographa californica nucleopolyhedrovirus (Ac NPV) in mammals. Toxicity tests in rats and guinea pigs, along with cell culture studies, showed no adverse effects or viral replication.
Area of Science:
- Virology
- Mammalian Toxicology
- Cell Biology
Context:
- Assessing the safety of insect-specific viruses for potential use in biological control or research is crucial.
- Autographa californica nucleopolyhedrovirus (Ac NPV) is a widely studied insect pathogen.
Purpose:
- To evaluate the potential toxicity and infectivity of Ac NPV in mammalian systems.
- To determine if Ac NPV can replicate in mammalian cells or cause adverse effects.
Summary:
- Mammalian toxicity studies were conducted on rats (oral and intravenous administration) and guinea pigs (skin irritation) using Ac NPV. No virus-induced changes were observed in any tested animals.
- Mammalian cell lines (human Hela Ohio, monkey Vero, rat REC) were exposed to Ac NPV through three blind passages. No signs of viral replication were detected.
- Cell-free extracts from these mammalian cultures were tested for infectivity in insect cell cultures, yielding negative results, further confirming the absence of viable Ac NPV.
Impact:
- Provides critical safety data regarding the use of Ac NPV, suggesting a low risk of mammalian infection.
- Informs risk assessment for biopesticide development and environmental release of baculoviruses.
- Supports the continued use of Ac NPV as a research tool without significant mammalian health concerns.