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Intranasal Delivery of mRNA Polyplexes via Rayleigh Breakup Aerosols: An In Vitro Method for Nasal Deposition and

Mazen Kafienah1, Ziheng Zheng2, Hao-Ying Li3

  • 1GKT School of Medical Education, Faculty of Life Sciences and Medicine, King's College London.

Journal of Visualized Experiments : Jove
|February 9, 2026
PubMed
Summary

A novel Rayleigh breakup nasal atomizer gently aerosolizes messenger RNA (mRNA) vectors, preserving their integrity for effective intranasal delivery and gene expression in respiratory disease treatments.

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