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Related Concept Videos

RACE - Rapid Amplification of cDNA Ends02:35

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Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
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Related Experiment Video

Updated: Feb 11, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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Amplification-free one-pot RNA detection by pairing CRISPR-Cas13a with cascade amplification circuit-driven DNAzyme

Xiaona Yin1, Ziyan Zhang1, Hao Luo1

  • 1Dermatology Hospital, Southern Medical University, Guangzhou, 510091, China; Guangzhou Key Laboratory for Sexually Transmitted Diseases Control, Guangzhou, 510091, China.

Analytica Chimica Acta
|February 9, 2026
PubMed
Summary
This summary is machine-generated.

A new RNA detection platform, RAPID (CRISPR-Cas13a with a cascade amplification circuit-driven DNAzyme), offers rapid, isothermal, point-of-care diagnostics. This CRISPR-based biosensor achieves sensitive and specific detection of bacterial and viral pathogens within 30 minutes.

Keywords:
CRISPR–Cas13aDNAzyme cleavageRNA detectionSignal amplificationToehold-mediated strand displacement

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Area of Science:

  • Molecular Biology
  • Biotechnology
  • Diagnostics

Background:

  • RNA detection is crucial for pathogen diagnosis, but current methods like RT-qPCR require complex equipment, limiting point-of-care (POC) applications.
  • Isothermal amplification offers simpler alternatives but faces challenges with contamination and efficiency.
  • Existing RNA diagnostic tools are often not suitable for resource-limited or on-site settings.

Purpose of the Study:

  • To develop a novel, isothermal, one-pot RNA detection platform for rapid and sensitive pathogen diagnosis.
  • To overcome the limitations of existing RNA-based assays, including the need for reverse transcription and complex instrumentation.
  • To create a versatile and programmable biosensing platform suitable for diverse infectious agents and adaptable to different readout formats.

Main Methods:

  • Developed RAPID (CRISPR-Cas13a with a cascade amplification circuit-driven DNAzyme), an isothermal biosensing platform.
  • Integrated CRISPR-Cas13a for target recognition with a DNA circuit for signal amplification, eliminating the need for reverse transcription and thermal cycling.
  • Enabled quantitative RNA detection within 30 minutes at 37°C, compatible with fluorescence (RAPID-Flu) and lateral flow assay (RAPID-LFA) readouts.

Main Results:

  • RAPID achieved quantitative RNA detection within 30 minutes at 37°C without sample pre-amplification.
  • The platform successfully detected bacterial (Treponema pallidum, Neisseria gonorrhoeae) and viral (herpes simplex virus) targets by reprogramming crRNAs.
  • Both RAPID-Flu and RAPID-LFA demonstrated a sensitivity of 5 fM per reaction, comparable detection limits, excellent specificity, and high concordance with clinical diagnoses.

Conclusions:

  • The RAPID platform offers a rapid, programmable, and visually interpretable solution for point-of-care RNA diagnostics.
  • Its flexibility, portability, and isothermal nature make it ideal for early diagnosis and on-site monitoring of infectious pathogens.
  • RAPID presents a significant advancement for nucleic acid-based diagnostics in diverse settings, addressing current limitations in speed, complexity, and accessibility.