Related Experiment Video
Updated: Feb 11, 2026

Protocols of 3D Bioprinting of Gelatin Methacryloyl Hydrogel Based Bioinks
Published on: December 21, 2019
3D alginate hydrogel microspheres with uniform micro-structure for cell culture and CVB3 infection
Tianyi Zhang1, Yuqing Xu1, Yiwei Sun1
1Department of Laboratory Medicine, School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, 212013, China.
Abstract:
An integrated microfluidic platform has been developed for the efficient generation of highly uniform alginate hydrogel microspheres (AHMs) encapsulating HeLa cells, enabling robust three dimensional (3D) cell culture and subsequent infection with Coxsackievirus B3 expressing enhanced green fluorescent protein (CVB3-eGFP). Our results demonstrate that AHMs support high cell viability and facilitated cell proliferation within a biomimetic 3D matrix. By systematically reducing the alginate concentration from 1.0% to 0.6%, we enhanced viral accessibility while maintaining microstructural integrity, thereby significantly improving CVB3-eGFP infection rates, as confirmed by fluorescence imaging and western blot analysis. This study establishes a tunable, reproducible, and physiologically relevant 3D model for studying virus-host interactions, with broad applications in antiviral drug screening and infectious disease modeling.
Related Concept Videos
Uniform Distribution
Two essential properties of this distribution are
Uniform Circular Motion
Non-uniform Circular Motion
For example, such...
Uniform Depth Channel Flow
Uniform Depth Channel Flow: Problem Solving
Electric Field of a Non Uniformly Charged Sphere
Consider a non-uniformly charged sphere, for which the density of charge depends only on the distance from a point in space and not on the direction. Such a sphere has a spherically symmetrical charge distribution. Here, the electric...

