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Synthesis of an In vivo MRI-detectable Apoptosis Probe
Published on: July 31, 2012
H2O2-Responsive Organochalcogen-Based Probes for 19F Chemical Shift-Switching MRI
Chenyu Peng1,2, Hongwu Liu2, Xueqi Wang2
1Department of Radiology, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
None:
Hydrogen peroxide (H2O2), a key reactive oxygen species, is overexpressed in metabolically dysregulated cells and involved in various disease processes. Herein, we present a class of organochalcogen-based complexes that serve as 19F MRI probes for H2O2 detection by exhibiting large 19F chemical-shift changes upon oxidation. Designed probes based on trifluoromethylselenide (CF3Se-) and trifluoromethyltelluride (CF3Te-) react rapidly with H2O2 under mild reaction conditions. The corresponding 19F chemical-shift changes reach up to 28 and 55 ppm, respectively, under our experimental settings. These substantial shift ranges enable 19F chemical-shift-switching MRI to specifically visualize H2O2-rich regions. Notably, the 19F MRI results of the Se-based analogue demonstrate that a detectable chemical-shift range can be achieved at biologically relevant H2O2 concentrations in a living mouse model of hind limb inflammation. This proof-of-concept study highlights the feasibility of constructing a visual imaging platform using organochalcogen-based 19F MRI probes with large chemical-shift responsiveness.
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