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Photobiomodulation Activated TGF-β1 Has Discrete Roles in Modulating Macrophage Responses to Bothrops jararacussu
Amanda Cabral David1,2, Mahmud Amin2, Ridham Varsani2
1Programa de Pós Graduação em Medicina, Universidade Nove de Julho, São Paulo, Brazil.
Photobiomodulation (PBM) therapy can mitigate harmful effects of Bothrops jararacussu snake venom (BjsuV) on macrophages. PBM counteracts venom-induced inflammation and cell damage, offering potential therapeutic benefits.
Area of Science:
- Biomedical Science
- Immunology
- Toxicology
Background:
- Bothrops jararacussu snake venom (BjsuV) causes severe tissue damage, with current antivenom therapy being insufficient for muscle injury.
- Photobiomodulation (PBM) is an emerging anti-inflammatory therapy with potential as an adjunct treatment.
Purpose of the Study:
- To investigate the effects of PBM on macrophage functions, specifically their response to BjsuV and the role of TGF-β1.
- To evaluate PBM's potential to counteract BjsuV-induced cellular damage and inflammation.
Main Methods:
- Macrophages were exposed to BjsuV, TGF-β1, a TGF-β inhibitor (SB431542), and PBM (660 nm red or 810 nm near-infrared laser).
- Macrophage survival, migration, and phagocytosis were assessed after various treatment combinations.
Main Results:
- BjsuV exposure reduced macrophage survival and migration but increased phagocytosis.
- PBM treatments significantly counteracted the negative effects of BjsuV on macrophage survival and migration.
- PBM modulated macrophage phagocytosis in response to BjsuV.
Conclusions:
- PBM demonstrates anti-inflammatory effects on macrophages, suggesting its therapeutic potential against venom-induced injuries.
- Understanding PBM's mechanisms in modulating macrophage signaling pathways can enhance future clinical applications.
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