Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Scope of Physics01:17

The Scope of Physics

53.5K
Physics is concerned with the interactions of energy, matter, space, and time, in order to discover the underlying mechanisms that underpin all phenomena. The word "physics" comes from the Greek word "phúsis", which means nature. Physics seeks to comprehend the natural world around us at its most fundamental level. It emphasizes the use of quantitative laws to do this, which could be valuable in other fields that want to push the performance boundaries of present...
53.5K
RNA-seq03:21

RNA-seq

12.1K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.1K
Depth Perception and Spatial Vision01:15

Depth Perception and Spatial Vision

2.1K
Depth perception is the ability to perceive objects three-dimensionally. It relies on two types of cues: binocular and monocular. Binocular cues depend on the combination of images from both eyes and how the eyes work together. Since the eyes are in slightly different positions, each eye captures a slightly different image. This disparity between images, known as binocular disparity, helps the brain interpret depth. When the brain compares these images, it determines the distance to an object.
2.1K
Chromatographic Resolution01:15

Chromatographic Resolution

2.2K
In chromatography, a solute moves through a chromatographic column and tends to spread, forming a Gaussian-shaped band. The longer the solute spends in the column, the broader the band becomes. The broadening can lead to overlaps within the column, affecting separation effectiveness.
The effectiveness of separation can be evaluated by determining the level of separation between two neighboring peaks in a chromatogram, which represents the individual components of a sample.
In chromatography,...
2.2K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
Racemic Mixtures and the Resolution of Enantiomers02:30

Racemic Mixtures and the Resolution of Enantiomers

21.8K
A racemic mixture, or racemate, is an equimolar mixture of enantiomers of a molecule that can be separated using their unique interaction with chiral molecules or media. Racemic mixtures are denoted by the (±)- prefix. This ‘optical rotation descriptor’ applies to the whole solution of a racemic mixture rather than a specific stereoisomer. Enantiomers typically have the same physical and chemical properties. Hence, they are not easily separable. However, enantiomers can exhibit...
21.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The developing tendon and enthesis are hypoxic and rely on hypoxia-inducible factor 1a during postnatal development.

Development (Cambridge, England)·2026
Same author

MilliMap: interactive closed-loop analysis for spatial omics.

bioRxiv : the preprint server for biology·2026
Same author

Imbalance of Ciliary Programs Drives Fibroblast Differentiation and Fibrotic Signaling in Systemic Sclerosis.

bioRxiv : the preprint server for biology·2026
Same author

Fluorescence-Guided Spatial Mapping of p21-Expressing Senescent Cells in Aged Liver at Single-Cell Resolution.

Biomolecules·2026
Same author

A Benchmark of Modern Statistical Phasing Methods.

bioRxiv : the preprint server for biology·2026
Same author

Short-, medium-, versus long-chain fatty acids: mechanisms of immunomodulation and disease pathogenesis.

Cellular & molecular immunology·2026
Same journal

Interplay between oxygen redox and interfacial stability of Li-rich positive electrodes in sulfide-based all-solid-state batteries.

Nature communications·2026
Same journal

Breaking dependence on melanisation imparts diversity to a dogmatic invasion strategy of phytopathogenic fungi.

Nature communications·2026
Same journal

Hydroxyl-rich nanocavities on perovskite enable nearly barrierless intramolecular hydrogen transfer for nitrate electroreduction to ammonia.

Nature communications·2026
Same journal

Household mobility responses to weather extremes in Kyrgyzstan.

Nature communications·2026
Same journal

Autonomous Motion Vision with Tri-bulk-heterojunctioned Organic Adaptation Transistor.

Nature communications·2026
Same journal

Tissue-adhesive hydrogel optical fiber for peripheral optogenetic neuromodulation.

Nature communications·2026
See all related articles

Related Experiment Video

Updated: Feb 12, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

18.9K

Seq-Scope-eXpanded: spatial omics beyond optical resolution.

Angelo Anacleto1, Weiqiu Cheng2, Qianlu Feng3,4

  • 1Department of Molecular & Integrative Physiology, University of Michigan, Ann Arbor, USA.

Nature Communications
|February 10, 2026
PubMed
Summary
This summary is machine-generated.

Seq-Scope-X enhances spatial transcriptomics (sST) by expanding tissues, achieving submicrometer resolution for whole-transcriptome and proteome profiling. This breakthrough reveals cellular compartment differences and dynamic metabolic roles in various tissues.

More Related Videos

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

12.6K
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

35.1K

Related Experiment Videos

Last Updated: Feb 12, 2026

Three-dimensional Optical-resolution Photoacoustic Microscopy
08:31

Three-dimensional Optical-resolution Photoacoustic Microscopy

Published on: May 3, 2011

18.9K
Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

12.6K
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

35.1K

Area of Science:

  • Molecular Biology
  • Genomics
  • Cell Biology

Background:

  • Sequencing-based spatial transcriptomics (sST) offers transcriptome-wide gene expression mapping.
  • Current sST methods lack the optical resolution of imaging techniques (200-300 nm).
  • Limitations in resolution hinder the detailed study of subcellular structures and gene expression patterns.

Purpose of the Study:

  • To develop an enhanced spatial transcriptomics method with submicrometer resolution.
  • To overcome the resolution limitations of existing sST techniques.
  • To enable ultra-high-resolution whole-transcriptome and proteome profiling in tissues.

Main Methods:

  • Introduction of Seq-Scope-X (Seq-Scope-eXpanded), a technique combining Seq-Scope with tissue expansion.
  • Physical enlargement of tissues to minimize transcript diffusion and increase spatial feature density.
  • Application in liver, brain, and colon tissues, and adaptation for spatial proteomics.

Main Results:

  • Seq-Scope-X achieves submicrometer resolution, surpassing conventional sST limitations.
  • Nuclear and cytoplasmic compartments were resolved in nearly all cells in liver tissue.
  • Widespread differences in nuclear vs. cytoplasmic transcriptome patterns were identified, suggesting dynamic metabolic switching in hepatocytes.
  • Successful application in brain and colon tissues, and adaptation for spatial proteomics in spleen and tonsil.

Conclusions:

  • Seq-Scope-X is a powerful platform for ultra-high-resolution whole-transcriptome and proteome profiling.
  • The method significantly expands spatial precision for studying cellular architecture and function.
  • Findings provide new insights into cellular heterogeneity and disease mechanisms at an unprecedented resolution.