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Updated: Feb 12, 2026
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
Published on: March 24, 2015
Exogenous-Label-Free Colorimetric/SERS Dual-Mode Immunosensing Platform Driven by Au NCs@Pt Nanozyme for
Qian-Jiao Qi1, Zhen Xu2, Yi-Fan Yu1
1College of Energy, College of Chemistry and Chemical Engineering, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Materials, School of Life Sciences, Institute of Artificial Intelligence, State Key Laboratory of Vaccines for Infectious Diseases, Xiang An Biomedicine Laboratory, Xiamen University, Xiamen 361005, China.
Abstract:
Early diagnosis of pancreatic cancer is critical for improving survival rates, yet conventional CA19-9 immunoassays and labeled surface-enhanced Raman scattering (SERS) methods are often limited by slow processing, complex fabrication, or instability. Herein, we report a rapid, exogenous-label-free colorimetric/SERS dual-mode sandwich immunoassay utilizing coral-like gold nanocores decorated with platinum clusters (Au NCs@Pt). This unique nanozyme architecture synergistically integrates high-density electromagnetic hotspots with efficient peroxidase-like activity to catalyze the oxidation of TMB. The resulting oxidized product (oxTMB) acts as an intrinsic Raman reporter, eliminating the need for exogenous labels and enabling catalysis-amplified signal reporting. The proposed platform achieves quantitative CA19-9 detection within 15 min, featuring a linear range of 1-200 IU/mL and an ultralow limit of detection (LOD) of 0.16 IU/mL. Clinical validation using 60 serum samples demonstrated 100% diagnostic accuracy (AUC = 1.00 in this cohort study). This strategy successfully overcomes the limitations of traditional SERS tags, offering a robust, ultrasensitive tool for the point-of-care screening of digestive system malignancies.
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