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Alhagi maurorum Extract Regulates the PI3K/AKT Cascade and Inhibits the Proliferation of the Lung Cancer Cell Line

Fereshteh Ramezani Zardanjani1, Hajar Yaghoobi2, Abbas Alibakhshi3

  • 1Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Advanced Biomedical Research
|February 11, 2026
PubMed
Abstract

Insights

Alhagi maurorum extract shows anticancer effects against non-small-cell lung cancer. This natural compound inhibits the PI3K/AKT pathway, offering potential for lung cancer treatment.

Area of Science:

  • Phytochemistry
  • Molecular Biology
  • Oncology

Background:

  • Non-small-cell lung cancer (NSCLC) remains a significant health concern.
  • Investigating natural compounds for anticancer properties is crucial for novel therapeutic development.

Purpose of the Study:

  • To evaluate the anticancer effects of hydroalcoholic *Alhagi maurorum* extract on NSCLC.
  • To identify the molecular targets and pathways affected by *A. maurorum* in lung cancer cells.

Main Methods:

  • Bioinformatic analysis of NSCLC signaling pathways (KEGG, EnrichR, STRING).
  • Molecular docking and dynamics simulations of *A. maurorum* compounds against pathway targets.
  • In vitro antioxidant (DPPH) and cytotoxic (MTT) assays on A549 lung cancer cells.
  • Real-time PCR for gene expression analysis related to KEGG pathways.

Main Results:

  • The PI3K-Akt signaling pathway was identified as a key pathway in NSCLC.
  • *A. maurorum* extract demonstrated cytotoxic effects on A549 cells with an IC50 of 277 μg/ml.
  • The extract exhibited antioxidant activity and specifically inhibited PTEN within the PI3K/AKT pathway.

Conclusions:

  • Hydroalcoholic *Alhagi maurorum* extract possesses anticancer properties against NSCLC.
  • The extract's mechanism involves the inhibition of the PI3K/AKT signaling pathway, potentially by targeting PTEN.
  • *A. maurorum* is a promising candidate for developing novel lung cancer therapeutics.
Keywords:
A549 cell lineAlhagi maurorumPTENlung cancer

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