Related Experiment Video
Updated: Feb 12, 2026

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
Published on: December 1, 2023
Long noncoding RNA LOC441461 drives cancer growth and is associated with poor clinical outcomes
Ching-Feng Cheng1,2, Ya-Ting Tu3, Yi-Ru Chen3
1Department of Pediatrics, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Taipei, Taiwan.
Objectives:
The role of LOC441461, a long noncoding RNA, differs in different cancer types, and its role in lung cancer remains unclear.
Materials And Methods:
We investigated the clinical significance of LOC441461 expression in lung cancer using bioinformatics analyses. To explore the biological function of LOC441461, we performed siRNA-mediated knockdown in lung cancer cells and evaluated its effects on cell proliferation, colony formation, apoptosis, and cell cycle progression.
Results:
LOC441461 was found to be significantly overexpressed in lung adenocarcinoma (LUAD), and LOC441461 overexpression was significantly associated with poor prognosis in patients with LUAD. LOC441461 knockdown significantly inhibited cell growth and induced a modest but statistically significant increase in apoptosis in lung cancer cells. According to a cell cycle analysis, LOC441461 knockdown induced G0/G1 arrest in A549 cells; increased p21 and p27 expression; and reduced the levels of CDK4 and the cyclins A2, B1, and D1, similar to p53-dependent regulation. By contrast, H1299 cells exhibited G2/M accumulation with no change in p21 and p27 levels, suggesting a p53-independent mechanism.
Conclusion:
Our findings indicate that high LOC441461 expression is correlated with worse prognosis in LUAD. These results support the potential of LOC441461 as a novel therapeutic and prognostic target in LUAD.
Related Concept Videos
Energy to Drive Translocation
Generally, polypeptides are unfolded by two distinct...
Predicting Reaction Outcomes
Outcomes of Glycolysis
Cellular respiration can occur aerobically (with oxygen) or anaerobically (without oxygen). In the presence of oxygen, cellular respiration starts with glycolysis and continues with pyruvate...
M-Cdk Drives Transition Into Mitosis
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
RNA Interference
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Guidelines for Writing Outcome
Patient outcomes reflect the patient's response to the goal rather than what the nurse aims to achieve. Terminology should be observable and measurable to avoid the reader's interpretation. The desired outcome should be realistic and achievable in the designated care timeframe. Expected outcomes should align with adjunctive therapies. The outcome should enhance care...

