Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Multiple Allele Traits01:49

Multiple Allele Traits

38.2K
The Concept of Multiple Allelism
38.2K
Next-generation Sequencing03:00

Next-generation Sequencing

98.8K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.8K
Lethal Alleles02:41

Lethal Alleles

18.2K
Agouti: A Lethal Allele
Lucien Cuénot discovered lethal alleles in 1905 while studying the inheritance of coat color in mice. The agouti gene is responsible for the color of the coat in mice. This gene codes for an agouti-signaling protein, which is responsible for melanin distribution in mammals. The wild-type allele gives rise to gray-brown coat color in mice, while the mutant allele gives rise to yellow coat color. In addition to coat color, the agouti gene is associated with the yellow...
18.2K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K
Sequences01:29

Sequences

295
Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
295
Sanger Sequencing01:57

Sanger Sequencing

774.9K
DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
774.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Three New Null HLA Alleles Were Identified of the Unrelated Bone Marrow Donors.

HLA·2026
Same author

Identification of Eleven Novel Synonymous Alleles Using Next Generation Sequencing.

HLA·2026
Same author

Ten Novel HLA Alleles Were Detected in Volunteer Bone Marrow Donors.

HLA·2026
Same author

Characterisation of Five Novel HLA Class II Alleles.

HLA·2026
Same author

Ten New HLA Class I Alleles Identified in Russian Bone Marrow Donors.

HLA·2026
Same author

Identification of the Novel HLA-DQB1*06:548 and Confirmation of the HLA-DRB1*14:284N Alleles.

HLA·2026

Related Experiment Video

Updated: Feb 12, 2026

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
08:07

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

10.6K

Next Generation Sequencing Identifies Two Novel Synonymous HLA Alleles.

Maria Loginova1,2, Igor Paramonov1, Sergei Bobovnik3

  • 1Federal State-Financed Scientific Institution: Kirov Research Institute of Hematology and Blood Transfusion Under the Federal Medical Biological Agency, Kirov, Russia.

HLA
|February 11, 2026
PubMed
Summary

Two new human leukocyte antigen (HLA) alleles, HLA-C*12:02:59 and DQB1*05:01:57, were discovered using advanced next-generation sequencing technology.

Keywords:
HLA‐C*12:02:59HLA‐DQB1*05:01:57NGSsilent mutation

More Related Videos

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

10.5K
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.9K

Related Experiment Videos

Last Updated: Feb 12, 2026

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
08:07

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

10.6K
RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level
11:04

RNA Next-Generation Sequencing and a Bioinformatics Pipeline to Identify Expressed LINE-1s at the Locus-Specific Level

Published on: May 19, 2019

10.5K
T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
08:59

T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing

Published on: January 12, 2021

8.9K

Area of Science:

  • Immunogenetics
  • Molecular Biology
  • Human Genetics

Background:

  • Human leukocyte antigen (HLA) genes are crucial for immune response.
  • Accurate HLA typing is essential for transplantation and disease association studies.
  • Novel HLA allele discovery refines our understanding of immune diversity.

Purpose of the Study:

  • To report the identification of two previously undescribed HLA alleles.
  • To contribute to the growing database of HLA polymorphism.

Main Methods:

  • Next-generation sequencing (NGS) was employed for high-resolution HLA typing.
  • Bioinformatic analysis was used to identify and characterize novel alleles.

Main Results:

  • Two novel HLA alleles were identified: HLA-C*12:02:59 and DQB1*05:01:57.
  • These findings expand the known repertoire of HLA genetic variations.

Conclusions:

  • The discovery of novel HLA alleles underscores the importance of continuous genetic surveillance.
  • Accurate identification of HLA alleles is vital for clinical and research applications.