Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

PCR01:32

PCR

238.5K
Overview
238.5K
DNA Base Pairing02:27

DNA Base Pairing

33.7K
Erwin Chargaff’s rules on DNA equivalence paved the way for the discovery of base pairing in DNA. Chargaff’s rules state that in a double-stranded DNA molecule,
33.7K
DNA Base Pairing02:27

DNA Base Pairing

33.0K
33.0K
Gene Families01:57

Gene Families

10.0K
Gene families consist of groups of genes proposed to have originated from a common ancestor. Typically these arise through events in which a gene or genes are mistakenly duplicated during cell division. Unlike their parent genes (which are subject to selection pressure to maintain function), these gene copies do not need to preserve their sequences and may evolve at a relatively faster rate.
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
10.0K
Proteins: From Genes to Degradation02:11

Proteins: From Genes to Degradation

14.6K
Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
Transcription is the synthesis of RNA...
14.6K
DNA-only Transposons02:57

DNA-only Transposons

17.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
17.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The Winthir Collection: An Identified Historical Skeletal Series From Munich, Germany.

American journal of biological anthropology·2026
Same author

Clinical application of whole exome and genome sequencing in pediatric neurodevelopmental disorders.

Clinical and experimental pediatrics·2026
Same author

Association of pre-transplant torque teno virus load with early post-transplant outcomes in lung transplant recipients: A multicenter cohort study.

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation·2026
Same author

Above- and belowground succession following multiple-tree mortality in <i>Pinus densiflora</i> forests.

Frontiers in plant science·2026
Same author

Serotype Distribution and Antimicrobial Resistance of <i>Salmonella</i> in Korea Between 2018 and 2022.

Annals of laboratory medicine·2025
Same author

Detection of <i>MYD88</i>L265P Mutation by Allele-Specific Polymerase Chain Reaction in Atypical Lymphoplasmacytic Lymphoma.

Clinical case reports·2025

Related Experiment Video

Updated: Feb 12, 2026

Optimized PCR-based Detection of Mycoplasma
06:01

Optimized PCR-based Detection of Mycoplasma

Published on: June 20, 2011

55.1K

Quantitative PCR-Based Optimization for Plasma DNA Quality Using Single- and Multi-Copy Reference Genes.

Jae Gyun Shin, Seung-Hwan Oh, Ja Young Lee

    Clinical Laboratory
    |February 11, 2026
    PubMed
    Summary

    This study developed a qPCR assay to assess plasma DNA quality by measuring DNA fragmentation. The small- to large-amplicon ratio effectively indicates DNA fragmentation, crucial for accurate cancer biomarker research.

    More Related Videos

    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
    11:40

    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

    Published on: November 14, 2018

    9.0K
    Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
    08:54

    Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

    Published on: November 5, 2020

    16.0K

    Related Experiment Videos

    Last Updated: Feb 12, 2026

    Optimized PCR-based Detection of Mycoplasma
    06:01

    Optimized PCR-based Detection of Mycoplasma

    Published on: June 20, 2011

    55.1K
    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
    11:40

    A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

    Published on: November 14, 2018

    9.0K
    Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications
    08:54

    Development and Testing of Species-specific Quantitative PCR Assays for Environmental DNA Applications

    Published on: November 5, 2020

    16.0K

    Area of Science:

    • Molecular Biology
    • Biochemistry
    • Genetics

    Background:

    • Improving circulating free DNA (cfDNA) purification is vital for accurate plasma DNA assessment.
    • Leukocyte DNA contamination can skew results in cancer biomarker research.
    • Quantitative analysis of housekeeping genes can serve as a quality indicator for cfDNA.

    Purpose of the Study:

    • To develop and assess quantitative PCR (qPCR) assays for evaluating plasma DNA quality.
    • To use housekeeping genes (LINE-1 and TOP1) as indicators of DNA fragmentation and contamination.
    • To optimize cfDNA purification methods for cancer biomarker research.

    Main Methods:

    • Selected LINE-1 (L1) and TOP1 genes for analysis.
    • Designed primer pairs to amplify short and long DNA fragments.
    • Utilized real-time quantitative PCR (qPCR) to determine amplicon copy numbers and calculate the small-to-large amplicon ratio (S/L).
    • Evaluated the impact of storage time and temperature on cfDNA extraction from K2EDTA tubes.

    Main Results:

    • The S/L ratio correlated with DNA fragmentation levels, with TOP1 showing higher sensitivity.
    • Plasma DNA extraction kit performance varied, with Qiagen yielding the highest S/L ratio.
    • Storage conditions significantly affected cfDNA quality, with S/L ratios decreasing after 3 days, especially at room temperature.

    Conclusions:

    • Developed qPCR assays to quantify plasma DNA fragmentation using single- and multi-copy reference genes.
    • The S/L amplicon copy number ratio is an effective measure of DNA fragmentation status.
    • This assay serves as a valuable tool for assessing plasma DNA quality in research settings.