Pseudenemella bisphenolivorans gen. nov., sp. nov., a Bisphenol A Degrading Actinobacterium Isolated from Waste...

A bisphenol A degrading actinobacterium, strain A6^T, was isolated from a waste digestion system in Chaozhou, China. Cells of strain A6^T were Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped, and forming yellow colonies on Reasoner's 2 A agar. Phylogenetic analyses based on the 16 S rRNA gene showed that strain A6^T shared 96.8% sequence similarity with Enemella dayhoffiae NML 130,396^T and formed a distinct lineage in the family Propionibacteriaceae in the phylogenetic trees. Genome-based phylogenetic analyses indicated that strain A6^T could be assigned as a novel genus in the family Propionibacteriaceae. The cellular components, including anteiso-C_15:0 (40.0%) as a predominant fatty acid, MK-9(H4) as the major respiratory quinone and a DNA G + C content of 67.2% supported strain A6^T as a member of the family Propionibacteriaceae. However, strain A6^T also contained C_11:0-3-OH (14.4%), iso-C_16:0 (11.3%), and C_11:0 (10.6%) as predominant fatty acids which distinguished it from other genera in the family Propionibacteriaceae. In addition, strain A6^T was negative in enzyme activity of esterase (C4), esterase lipase (C8), aminase of leucine, and negative in the utilization of most carbon sources, which were different from its close related genera in the family Propionibacteriaceae and supported it as a novel genus. Therefore, the name Pseudenemella bisphenolivorans gen. nov., sp. nov. is proposed. The type strain is A6^T (= GDMCC 1.3237^T =KCTC 92291^T).