Related Experiment Video

Updated: Feb 13, 2026

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells
12:43

Generation of a Novel Dendritic-cell Vaccine Using Melanoma and Squamous Cancer Stem Cells

Published on: January 6, 2014

12.2K

Redefining Dendritic Cell Vaccines: Synergistically Co-priming DC and B Cells With Nanoparticles Loading Whole Cell

Xiangxiang Xu1,2,3,4,5, Xianlan Chen1,2, Jin Wang1,2

  • 1Department of Pharmaceutics, College of Pharmaceutical Sciences, Soochow University, Suzhou, P. R. China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|February 11, 2026
PubMed

Dendritic cells (DC) play core roles in inducing antigen-specific T cells. However, limited effectiveness hinders their applications as vaccines. To improve the efficacy of traditional DC vaccines, this study optimized three aspects: tumor antigens, cell sources and co-incubating molecules, and thus proposed a new DC-BC vaccine: co-priming B cells and DC at a precise ratio. The therapeutic efficacy was significantly improved by co-incubating DC and B cells. Regarding tumor antigens, utilizing nanoparticles loading whole-tumor lysates performed better than utilizing nanoparticles loading multiple neo-antigens, or nanoparticles loading only water-soluble lysates, or free whole-tumor lysates. Moreover, adding IL-15 and αPD-L1 antibody further bettered DC-BC vaccines. The optimal DC-BC vaccines showed excellent therapeutic efficacy with a 100% response rate and could cure most tumor-bearing mice on several different cancer models, including melanoma, lung cancer and orthotopic pancreatic cancer. The mechanism investigation demonstrated that several molecules, including Ticam1 (TRIF), Traf3, Mavs, and Ifnar2, were involved in promoting APC maturation and improving therapeutic efficacy of vaccines by activating innate immune pathways (TLR/NLR/RLR). In summary, this study provides a new DC-BC vaccine that has much better therapeutic efficacy and explores the underlying mechanism of why co-incubating DC and B cells improved the therapeutic efficacy.

Keywords:
B cellDC‐BC vaccinecancer immunotherapycancer vaccinedendritic cell

More Related Videos

Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells12:54

Determination of Vaccine Immunogenicity Using Bovine Monocyte-Derived Dendritic Cells

Published on: May 19, 2023

2.4K
Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination11:07

Whole-animal Imaging and Flow Cytometric Techniques for Analysis of Antigen-specific CD8+ T Cell Responses after Nanoparticle Vaccination

Published on: April 29, 2015

13.8K

Related Concept Videos

Vaccinations01:51

Vaccinations

Overview
51.9K
DC Generator01:19

DC Generator

An alternator converts mechanical energy into electrical energy that varies sinusoidally, resulting in AC current. Meanwhile, a DC generator converts mechanical energy into electrical energy, which are DC pulses with the same polarity. The construction of a DC generator is similar to that of an alternator, except that the pair of slip rings is replaced by a single split ring, also called a commutator. The commutator functions like a periodic rotary switch; it changes the contacts with the...
2.1K
DC Battery01:21

DC Battery

A conductor needs to be a component of a path that creates a closed loop or full circuit to have a continuous current flowing through it. A current starts to flow if an electric field is created inside an isolated conductor that is not part of a full circuit. The conductor quickly develops a net positive charge at one end and a net negative charge at the other. These charges generate an electric field opposite the direction of the applied electric field, which reduces the current. Eventually,...
1.3K
Cancer Vaccines01:30

Cancer Vaccines

Cancer treatment vaccines are a rapidly evolving field that offers a promising approach to immunotherapy. Unlike traditional vaccines that prevent diseases, cancer treatment vaccines are designed to treat existing cancers by stimulating the immune system to recognize and attack cancer cells.
Cancer vaccines come in two categories: preventive (prophylactic) and treatment (active). Preventive vaccines, such as the Human Papillomavirus (HPV) vaccine, protect against viruses that cause certain...
1.1K
Fast Decoupled and DC Powerflow01:24

Fast Decoupled and DC Powerflow

The fast decoupled power flow method addresses contingencies in power system operations, such as generator outages or transmission line failures. This method provides quick power flow solutions, essential for real-time system adjustments. Fast decoupled power flow algorithms simplify the Jacobian matrix by neglecting certain elements, leading to two sets of decoupled equations:
766
Antigen Presenting Cells01:22

Antigen Presenting Cells

The immune system is a complex network of cells and molecules that protects the body from foreign invaders. T cells, a type of white blood cell, play a crucial role in this process. They recognize and attack foreign substances, such as pathogens, that enter the body.
T cells require the help of antigen-presenting cells (APCs), which process foreign antigens into smaller fragments that can be recognized by T cells. These APCs are highly specialized cells that efficiently internalize antigens...
3.4K